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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名:
NK1.1 Monoclonal Antibody (PK136), APC, eBioscience™
- 浓度:
0.2 mg/mL
- 应用范围:
见产品说明书
- 宿主:
Mouse
- 供应商:
嵘崴达
- 库存:
需确认
- 保质期:
见产品说明书
- 抗原来源:
见产品说明书
- 适应物种:
见产品说明书
- 标记物:
见产品说明书
- 是否单克隆:
单克隆
- 保存条件:
4° C
- 亚型:
IgG2a
- 规格:
50 ug
NK1.1 Monoclonal Antibody (PK136), APC, eBioscience™
货号 17-5941-81产品描述
Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. No. 14-5971) should be used. Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.
Applications Reported: PK136 has been reported for use in flow cytometric analysis.
Applications Tested: The PK136 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes and can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
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- 作者
- 内容
- 询问日期
文献和实验Intracellular Cytokine Staining Protocol
Natural T, NK+ NK1.1 PK136 14-5941 small subset CD3+DX5+ or CD3+NK1.1+ CD49b/DX5 DX5 14-5971
Fluorochrome Absorption and Emission Spectra
light at 488 nm for three-color flow cytometric analysis (Fig. 1). BD Biosciences Pharmingen also offers monoclonal antibodies and proteins conjugated to allophycocyanin (APC) and avidin conjugated to Texas Red™. Both of these dyes
Fluorochrome Absorption and Emission
for three-color flow cytometric analysis (Fig. 1). BD Biosciences Pharmingen also offers monoclonal antibodies and proteins conjugated to allophycocyanin (APC) and avidin conjugated to Texas Red™. Both of these dyes are useful in experiments
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