IFN gamma Human Uncoated ELISA

IFN gamma Human Uncoated ELISA Kit

货号 88-7316-77

产品描述
The Human Interferon gamma (IFNγ) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IFNγ. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.

原理方法

ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

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