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- eBioscience
- 14-1079-80
- 原装进口
- 2025年07月13日
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), eBioscience™
- 浓度：
0.5 mg/mL
- 宿主：
Mouse
- 供应商：
嵘崴达
- 保质期：
见产品说明书
- 抗原来源：
见产品说明书
- 适应物种：
见产品说明书
- 库存：
需确认
- 标记物：
见产品说明书
- 是否单克隆：
单克隆
- 保存条件：
见产品说明书
- 亚型：
IgG1
- 规格：
25ug
货号 14-1079-80
产品描述
Description: The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a highly glycosylated protein of approximately 110kDa. It is predominantly expressed intracellularly in the lysosomal/endosomal membrane in nearly all cells. CD107a is transiently expressed on the cell surface of degranulating cytolytic T cells, and is also upregulated on the surface of activated platelets and some cancer cells.
Applications Reported: Purified anti-human CD107a (LAMP-1) has been reported for use in flow cytometric analysis, immunohistochemistry, immunocytochemistry, and immunoblotting. It has also been reported for use in surface staining in a flow cytometric based degranulation assay. (Fluorochrome conjugated eBioH4B4 (H4B4) is recommended for use in flow cytometry.).
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文献和实验Isolation of Untouched Human CD8+ T Cells from Peripheral Blood Mononuclear Cells (PBMC)
实验原理 A mixture of biotinylated monoclonal antibodies against the non-CD8 T cells is added to the starting sample and allowed to bind to the cells. Depletion MyOne™ SA Dynabeads® are added and will bind to the antibody
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
/Western blotting assays. Another factor important in achieving optimal results is the degree of sensitivity of cells to the antibody-induced apoptosis. As an example, CD95 (Fas)-induced apoptosis of human Jurkat cell line by monoclonal antibody EOS
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