MAP1A antibody

MAP1A antibody

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  • ¥780 - 2200
  • 康朗生物
  • kl-1847R
  • 中国/美国/德国
  • 2025年07月12日
  • IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500
  • Rabbit
  • Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,
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    • 详细信息
    • 技术资料
    • 供应商

      上海康朗生物科技有限公司

    • 库存

      大量

    • 目录编号

      kl-1847R

    • 克隆性

      多克隆

    • 抗原来源

      Rabbit

    • 保质期

      12个月

    • 抗体英文名

      MAP1A antibody

    • 抗体名

      微管相关蛋白1A抗体

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,

    • 免疫原

      KLH conjugated synthetic peptide derived from human MAP1A heavy chain:2651-1750/3014

    • 亚型

      IgG

    • 形态

      冻干粉或液体

    • 应用范围

      IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500

    • 浓度

      1mg/ml

    • 保存条件

      -20 °C

    • 规格

      50ul 100ul 200ul

    MAP1A antibody
    中文名称 微管相关蛋白1A抗体
    别    名 MAP 1A; MAP-1A; MTAP1A; MAP 1L; MAP1L; MAP1A heavy chain; Map1a; MAP1A_HUMAN; Microtubule associated protein 1 like; Microtubule Associated Protein 1A; Microtubule-associated protein 1A; MTAP1A; Proliferation related protein p80; Proliferation-related protein p80; AI853608; Map1a; moth1; Mtap-1; Mtap1.  
    规格价格 50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 50ul  100ul  200ul
    研究领域 肿瘤  细胞生物  免疫学  神经生物学  信号转导  细胞凋亡  激酶和磷酸酶  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, 
    产品应用 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 326kDa
    细胞定位 细胞浆 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human MAP1A heavy chain:2651-1750/3014 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1A heavy chain and LC2 light chain. Expression of this gene is almost exclusively in the brain. Studies of the rat microtubule-associated protein 1A gene suggested a role in early events of spinal cord development. [provided by RefSeq, Jul 2008]

    Function:
    Structural protein involved in the filamentous cross-bridging between microtubules and other skeletal elements.

    Subunit:
    3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. Interacts with TIAM2. Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG4. Binds to CSNK1D.

    Subcellular Location:
    Cytoplasm, cytoskeleton (Probable).

    Tissue Specificity:
    Brain

    Post-translational modifications:
    Phosphorylated by CSNK1D. 
    LC2 is generated from MAP1A by proteolytic processing.

    Similarity:
    Belongs to the MAP1 family.

    SWISS:
    P78559

    Gene ID:
    4130

    Database links:

    Entrez Gene: 4130 Human

    Entrez Gene: 17754 Mouse

    Entrez Gene: 25152 Rat

    Omim: 600178 Human

    SwissProt: P78559 Human

    SwissProt: Q9QYR6 Mouse

    SwissProt: P34926 Rat

    Unigene: 194301 Human

    Unigene: 619338 Human

    Unigene: 36501 Mouse

    Unigene: 11402 Rat

    Unigene: 41412 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片 MAP1A antibody
    Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP1A) Polyclonal Antibody, Unconjugated (bs-1847R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    MAP1A antibody
    Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; 
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
    Incubation: Anti-MAP1A Polyclonal Antibody, Unconjugated(bs-1847R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    MAP1A antibody
    Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
    Incubation: Anti-MAP1A Polyclonal Antibody, Unconjugated(bs-1847R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    MAP1A antibody
    Blank control (blue line): U251 (blue). 
    Primary Antibody (green line): Rabbit Anti- MAP1A antibody (bs-1847R) 
    Dilution: 1μg /10^6 cells; 
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
    Dilution: 1μg /test. 
    Protocol
    The cells were fixed with 2% paraformaldehyde (10 min , then permeabilized) with 90% ice-cold methanol for 20 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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