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Q SEPHAROSE HIGH PERF. 75 ML
上海嵘崴达实业有限公司,专业经营生命科学领域的科研试剂、仪器和实验室消耗品,
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| 17101401 | Q SEPHAROSE HIGH PERF. 75 ML |
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文献和实验BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
1、A smear (rather than a single colony) of BAC colonies are picked and transferred into a 12X75 mm Falcon tube containing 3 ml of LB medium (10 g Bacto-Tryptone, 5 g Bacto-yeast extract, and 10 g NaCl in 1 L H2O, autoclaved) with appropriate
Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period
100. Add 10 ml of 1 M Tris, 109.54 gr of sucrose, 1.01 gr MgC12, adjust pH to 8.0 and finally add 10 ml of Triton X-100 to 800 ml of distilled water, and make up to 1 liter with distilled water. Autoclave at 15 p.s.i. for 10 min. Sugars at high
Sepharose 4B-CL Chromatography BAC DNA Purification for Transgenic Production
in a high yield of BAC DNA. We use a Pasteur pipet to both "rub" the cell pellet on the side of the Corex tube and to suspend the cells by repeated aspiration. 5. Immediately add to each combined cell pellet from a 50 ml culture 4 mls of 0.2 M sodium
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