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嵘崴达
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ETFE TUBING, 2M/ID0.5/OD1/17
上海嵘崴达实业有限公司,专业经营生命科学领域的科研试剂、仪器和实验室消耗品,
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| 18112096 | ETFE TUBING, 2M/ID0.5/OD1/17 |
| 18112116 | FEP TUBING, 3M/ID1/16/OD1/8 |
| 18112117 | TUBING CONNECTOR 1/8in |
| 18112118 | FERRULE FOR 1/8in TUBING |
| 18112135 | FLOW RESTRICTOR,FR-902 |
| 18112136 | ETFE TUBING,2M/ID0,25/OD1/17 |
| 18112164 | TUBING KIT I.D. O,5 MM |
| 18112165 | TUBING KIT, I.D. 1,0 MM |
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文献和实验Chick Chorioallantoic Membrane (CAM) Assay
for gross evaluation. For injection, borosilicate glass capillaries (OD 1.0 mm, ID 0.75 mm; Sutter Instrument Company, Novato, CA) were prepared with P-87 micropipette puller (Sutter Instrument Company). Needles were connected with Tygon tubing (ID 1/32
Myosin Light Chain Preparation
in the buffer.Spin in the J-6 for 10 minutes at 1000 rpm. Repeat for a total of 4 washes. 17.Pack the DE52 into the 5½ cm ID column in the cold room. 18.When all of the DE52 has been put in the column and has packed somewhat,take a 10 sample of the eluting
LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
ratio.Dilute 1:40 with the dialysis buffer and read the OD at 495 nm. D/P = {OD495 x 41 / 60,000} / {(mg/ml)/ 43,000} 8.Dilute to 3-5 mg/ml with the dialysis buffer.Calculate total mg of actin.Drop freeze in liquid N2 after dissolving 2 mg sucrose
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