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- Abbexa
- 英国
- abx184643
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
参照说明书
- 英文名：
Ammonium cerium(IV) sulfate dihydrate
- 库存：
1
- 供应商：
北京博蕾德生物科技有限公司
- 规格：
25 g//100 g//
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文献和实验Replication timing by density transfer
C]- acetate or glucose 0.01% (w/v) [15 N]- ammonium sulfate Amino acids/supplements (as required, added to the same concentration as in normal synthetic medium) --dissolved in "-N" medium and filtered
Large scale nuclear extract preparation
pellets in nuclear lysis buffer (5ml/pellet).Perform one stroke with Dounce homogenizer 3 Add lysis buffer to 40ml and 2ml of 4M ammonium sulfate and agitate for 30-60min at 4℃ IV.Reprecipitate nuclear proteins 1.Centrifuge lysates in 55.2Ti rotor (35
Large scale nuclear extract preparat
buffer (5ml/pellet).Perform one stroke with Dounce homogenizer 3 Add lysis buffer to 40ml and 2ml of 4M ammonium sulfate and agitate for 30-60min at 4℃ IV.Reprecipitate nuclear proteins 1.Centrifuge lysates in 55.2Ti rotor (35,000rpm,60min,1℃ or 60Ti
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