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文献和实验DAY 1 1. Coat Nunc immuno-module plates overnight, in usual manner. 2. Set up competition assay between antibody and competing substance :- Prepare a 1:2 serial dilution of the competitive substance from 480 to 0.4 ng/ml
DAY 1 1. Coat Nunc immuno-module plates overnight, in usual manner. 2. Set up competition assay between antibody and competing substance :- Prepare a 1:2 serial dilution of the competitive substance from 480 to 0.4 ng/ml. Add 50
from control and immunized mice 2. distribute 2.5x106 platelets per well on F-bottomed MaxiSorp Nunc-Immuno microplates (GibcoBRL, Roskilde, Denmark), centrifuge at 3000 rpm, 7 min, 10ѓC. 3. fix with PBS-PF 2 % for 5 min, RT 4. wash 3 times in PBS-tween 20
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