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文献和实验E. Z. N.A.TM X-press Plasmid Protocol
at maximum speed (13,000 x g) for 1 minute to dry the column. 14. Transfer the X-press spin column into a new 1.5 ml centrifuge tube. Add 50 uL Elution Buffer (10mM Tris Hcl pH 8.5) directly onto the center of the membrane. 15. Centrifuge
E-Z 96 X-press Plasmid Vacuum Protocol
selective antibiotic. Incubate 14-16 hours at 37℃ 600 with vigorous shaking until an OD of 2.0-4.0 is achieved. 2. Pellet 1-2 mL bacterial culture in a 2.0 ml 96-well deep-well plate by centrifugation at 3000 x g for 15 min at room temperature.
Clonality - X Chromosome Inactivation Assay
Clonality - X Chromosome Inactivation Assay This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research
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