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文献和实验Large-Scale Isolation of Plasmid DNA
by inverting rapidly. Let stand on ice for 5 minutes. 7. Transfer to 2 Oak Ridge tubes and centrifuge at 11,000 rpm for 20 minutes at 4 degrees. The pellet formed in this step will contain most of the chromosomal DNA, SDS-protein complexes
Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831
Preparation of Competent Cells (from PMB)
and reading in a plastic cuvette (discard cells afterward): If flanking arm flasks are accessible, use them for measuring O.D. 3.Spin the cells in sterile Oak Ridge Tube (5 K, 5 min, 4 ℃) and resuspend in 1 ml PMB #A. Then add another 9 ml PMB #A and spin
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