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- 详细信息
- 技术资料
- 供应商:
上海康朗生物科技有限公司
- 库存:
大量
- 目录编号:
kl-14387R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
12个月
- 抗体英文名:
DNAJC21 antibody
- 抗体名:
DNAJC21蛋白抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Zebrafish, Sheep,
- 免疫原:
KLH conjugated synthetic peptide derived from human DNAJC21:11-120/531
- 亚型:
IgG
- 形态:
冻干粉或液体
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500
- 浓度:
1mg/ml
- 保存条件:
-20 °C
- 规格:
100ul 200ul
DNAJC21 antibody
| 中文名称 | DNAJC21蛋白抗体 |
| 别 名 | DJC21_HUMAN; DnaJ homolog subfamily A member 5; DnaJ homolog subfamily C member 21; DNAJA5; dnajc21; GS3; JJJ1; Protein GS3. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 神经生物学 信号转导 转运蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Zebrafish, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 62kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DNAJC21:11-120/531 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: With the presence of the J domain defining a protein as a member, the DnaJ family has evolved with diverse cellular localization and functions and is one of the largest chaperone families. DnaJ heat-shock-induced proteins are derived from the bacterium Escherichia coli and are controlled by the htpR regulatory protein. DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis. Members of this family contain cysteine-rich regions composed of zinc fingers that form a peptide-binding domain responsible for chaperone function. DnaJ proteins are important mediators of proteolysis and are involved in the regulation of protein degradation, exocytosis and endocytosis. DNAJC21 (DnaJ homolog subfamily C member 21), also known as DNAJA5 or JJJ1, is a 531 amino acid protein that contains two C2H2-type zinc fingers and one J domain. Expressed in placenta, pancreas, kidney and brain, DNAJC21 may be a co-chaperone for HSP 70. Function: May act as a co-chaperone for HSP70. Tissue Specificity: Brain, placenta, kidney and pancreas. Similarity: Contains 2 C2H2-type zinc fingers. Contains 1 J domain. SWISS: Q5F1R6 Gene ID: 134218 Database links: Entrez Gene: 134218 Human SwissProt: Q5F1R6 Human Unigene: 131887 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Sample: 293T (human)Cell Lysate at 40 ug Cerebrum (mouse) Lysate at 40 ug kidney (mouse) Lysate at 40 ug pancreas (mouse) Lysate at 40 ug Primary: Anti- DNAJC21(bs-14387R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62kD Observed band size: 62kD ![]() Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DNAJC21) Polyclonal Antibody, Unconjugated (bs-14387R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
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DNAJC21 antibody
¥1380 - 2200











