脂蛋白脂酶-11145991103

Lipoprotein Lipase, chemically modified
from Pseudomonas species, lyophilizate 11 145 991 103
Enzyme that hydrolyzes triglycerides into three free fatty acids and glycerol.
Application
Use Lipoprotein lipase in diagnostic tests for the determination of triglycerides
together with Glycerol Kinase, Catalog Nos. 10 539 937 103 or 11 499 530 103
and Glycerol-3-phosphate Dehydrogenase, Catalog Nos. 11 654 730 103 or 11
582 003 103.
Benefits
Take advantage of the enhanced liquid stability of this enzyme. ■
Rely on the proven diagnostic quality of this product. ■
EC 3.1.1.34
Properties
Nomenclature: Triacylglycero-protein acylhydrolase
Molecular weight: 47 kD
Effectors: Hg 2+ , Ag + , Cr 2+ , Sn 2+ , Cu 2+ and ionic detergents inhibit. Mg 2+ ,
sodium cholate and BSA stabilize the enzyme. 4-Chloromercuribenzoate (2
mmol/l), monoiodoacetate (2 mmol/l), NaF (20 mmol/l), NaN 3 (20 mmol/l),
EDTA (5 mmol/l) and 2-phenanthroline (2 mmol/l) do not affect the enzyme
activity while SDS (0.1% (w/v)) is inactivating.
pH optimum: 7.5 (see figure)
pH stability: 6.0-10.0 (see figure)
Thermal stability: Up to +50°C (see figure)
Specificity: Lipoprotein Lipase has both lipolytic and sterol ester hydrolytic
activities. It hydrolyzes triacylglycerols in chylomicrons, lipoproteins and diacyl-
glycerols. With human plasma as substrate triglycerides are hydrolyzed more
rapidly than cholesterol esters. The effects of pH and ionic strength on the en-
zymatic activity are somewhat different between the hydrolysis of triglyceride
and of cholesterol ester depending on the different states of these substrates
in the plasma or the transfer of the reaction products at the interface of sub-
strates.
Lipolytic activity (Substrate, Number of C-atoms to number of double bonds,
Relative rate):
olive oil: 94%
triolein (18:1): 100%
tripalmitin (16:0): 2%
trimyristin (14:0): 7%
trilaurin (12:0): 4%
tricaprin (10:0): 17%
tricaprylin (8:0): 64%
tricaproin (6:0): 2%
tributyrin (4:0): 2%
tripropionin (3:0): 2%
triacetin (2:0): 1%
Remark: Chemically modified Lipoprotein Lipase (LPL) is more hydrophilic
than native LPL. Carryover effect is therefore reduced.
Specification
Appearance: Brownish lyophilizate
Solubility: Clear, brown solution in water (c=50 mg/ml)
Activity (+25°C, cholesterol oleate): ≥10 U/mg lyophilizate
Contaminants (expressed as percentage of Lipoprotein Lipase activity):
ATPase: ≤0.005
Catalase: ≤1.0
Glycerokinase: ≤0.001
Hexokinase: ≤0.005
''NADH oxidase'': ≤0.001
Uricase: ≤0.005
Stability: At +2 to +8°C within specification range for 12 months. Store dry.