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Nanoprobes
上海钰博生物科技有限公司
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国别:美国Nanoprobes
1.4nm的纳米金探针 授权签约代理
Nanoprobes公司专业生产免疫金标记和免疫测定试验产品。公司建立之初即研发用于检测生物分子的最灵敏的试剂和技术。其独特的金标技术使用化学交联的金属和纳米颗粒团簇作为标记。与胶体金颗粒静电吸附在抗体和蛋白质上的传统免疫金探针不同,本公司的金标是与生物分子在特定位点交联的不带电荷的分子。这使我们的探针具有胶体金不具备的范围和灵活性。
产品:
应用:
可替代HRP底物,用于原位杂交/免疫组化/电镜/关联显微系统/免疫斑点/蛋白杂交等
优点:
染色清晰度优于传统生色或荧光底物,永久不褪色,不扩散
可检测内源单拷贝基因(原位杂交中)!超高灵敏度
高分辨率
背景接近零
无需更多处理,染色即在电镜下可见
免疫组化:DAB(左)EnzMet(右)染HER2扩增的组织
品名 |
产品描述 |
货号 |
规格 |
EnzMet™ IHC / ISH HRP Detection Kit |
专为免疫组化和原位杂交优化的HRP检测试剂盒 |
6001 |
150张载玻片 |
EnzMet™ Western Blot HRP Detection Kit |
在蛋白杂交中获得最高灵敏度的EnzMet |
6002 |
100ml |
EnzMet™ for General Research Applications |
普通实验用的EnzMet |
6010 |
45ml |
References:
左:1.8nm Ni-NTA-纳米金与his标签蛋白相互作用右:用Ni-NTA-纳米金标记6xhis腺病毒Knob蛋白,未染色的扫描电镜照片
left: Structure of Ni-NTA-Nanogold® showing interaction with Interaction with a His-tagged protein; right: Knob protein from adenovirus cloned with 6x-His tag, labeled with Ni-NTA-Nanogold, column purified from excess gold, and viewed in the scanning transmission electron microscope (STEM) unstained (Full width approximately 245 nm).
上:5nm Ni-NTA-纳米金的结构,显示金属螯合物与his标签蛋白结合
下:5nm Ni-NTA-纳米金的扫描电镜图,平均直径5.11±0.84nm。
Top: Structure of NTA-Ni(II)-5 nm Nanogold®, showing the binding of the incorporated metal chelate to a His-tagged protein; distance from the gold particle surface to the His tag is estimated to be 1.5 nm. Above: Transmission electron micrograph of 5 nm NTA Nanogold: average diameter 5.11±0.84nm.
品名 |
产品描述 |
货号 |
规格 |
1.8 nm Ni-NTA-Nanogold® |
比抗体小,更易渗透入组织,定位和检测细胞、组织或蛋白复合物中的his标签蛋白。 |
2080 |
10uM 30ml |
5 nm Ni-NTA-Nanogold® |
目标量达一定程度时,无需银增强/金增强可直接用电镜观察 |
2082 |
0.5uM 3ml |
相关产品:
GoldEnhance LM/Blot (GELM) |
金增强试剂,用于光镜样品 |
2112 |
Initiator/Moderator/Activator/Buffer |
GoldEnhance EM(GEEM) |
金增强试剂,用于电镜样品 |
2113 |
Initiator/Moderator/Activator/Buffer |
HQ Silver |
用于纳米金的质量最佳的增强试剂,均匀显影,极好的保持结构,特别适合电镜,光敏感 |
2012 |
Initiator/Moderator/Activator |
Li Silver |
纳米金的银增强,用于电镜、光镜、凝胶、杂交,光不敏感 |
2013 |
Initiator/Enhancer |
References:
1. Kollman, J. M.; Zelter, A.; Muller, E. G.; Fox, B.; Rice, L. M.; Davis, T. N., and Agard, D. A.: The Structure of the gamma-Tubulin Small Complex: Implications of Its Architecture and Flexibility for Microtubule Nucleation. Mol. Biol. Cell, 19, 207-215 (2008).
2. Adami, A.; Garcia-Alvarez, B.; Arias-Palomo, E.; Barford, D., and Llorca, O.: Structure of TOR and its complex with KOG1. Mol. Cell., 27509-516 (2007).
3. Balasingham, S. V.; Collins, R. F.; Assalkhou, R.; Homberset, H.; Frye, S. A.; Derrick, J. P., and Tonjum, T.: Interactions between the Lipoprotein PilP and the Secretin PilQ in Neisseria meningitidis. J. Bacteriol., 189, 5716-5727 (2007).
4. Jiang, Z. G.; Simon, M. N.; Wall, J. S., and McKnight, C. J.: Structural analysis of reconstituted lipoproteins containing the N-terminal domain of apolipoprotein B. Biophys. J., 92, 4097-4108 (2007).
5. Pye, V. E, Beuron, F, Keetch, C. A, McKeown, C, Robinson, C. V, Meyer, H. H, Zhang, X, and Freemont, P. S.: Structural insights into the p97-Ufd1-Npl4 complex. Proc. Natl. Acad. Sci. USA, 104, 467-472 (2007).
6. Promnares, K.; Komenda, J.; Bumba, L.; Nebesarova, J.; Vacha, F., and Tichy, M.: Cyanobacterial Small Chlorophyll-binding Protein ScpD (HliB) Is Located on the Periphery of Photosystem II in the Vicinity of PsbH and CP47 Subunits. J. Biol. Chem., 281, 32705-32713 (2006).
7. Collins, R. F.; Beis, K.; Clarke, B. R.; Ford, R. C.; Hulley, M.; Naismith, J. H.; and Whitfield, C.: Periplasmic protein-protein contacts in the inner membrane protein Wzc form a tetrameric complex required for the assembly of Escherichia coli group 1 capsules. J. Biol. Chem.,281, 2144-2150 (2006).
8. Wolfe, C. L.; Warrington, J. A.; Treadwell, L., and Norcum, M. T.: A three-dimensional working model of the multienzyme complex of aminoacyl-tRNA synthetases based on electron microscopic placements of tRNA and proteins. J. Biol. Chem., 280, 38870-38878 (2005).
9. Bumba, L.; Tichy, M.; Dobakova, M.; Komenda, J., and Vacha, F.: Localization of the PsbH subunit in photosystem II from the Synechocystis 6803 using the His-tagged NiNTA Nanogold labeling. J. Struct. Biol., 152, 28-35 (2005)
10. Collins, R. F.; Frye, S. A.; Balasingham, S.; Ford, R. C.; Tonjum, T., and Derrick, J. P.: Interaction with type IV pili induces structural changes in the bacterial outer membrane secretin PilQ. J. Biol. Chem., 280, 18923-18930 (2005).
11. Chatterji, A.; Ochoa, W. F.; Ueno, T.; Lin T., and Johnson, J. E.: A virus-based nanoblock with tunable electrostatic properties. Nano Lett.,5, 597-602 (2005).
12. Buchel, C.; Morris, E.; Orlova, E., and Barber, J.: Localisation of the PsbH subunit in photosystem II: a new approach using labelling of His-tags with a Ni(2+)-NTA gold cluster and single particle analysis. J. Mol. Biol., 312, 371-379 (2001).
13. Hainfeld, J. F.; Liu, W.; Halsey, C. M. R.; Freimuth, P., and Powell, R. D.: Ni-NTA-Gold Clusters Target His-Tagged Proteins. J. Struct. Biol., 127, 185-198 (1999).
14. Hainfeld, J. F.; Liu, W.; Joshi, V., and Powell R. D.: Nickel-NTA-Nanogold Binds his-Tagged Proteins. Microsc. Microanal., 8, (Suppl. 2: Proceedings) (Proceedings of Microscopy and Microanalysis 2002); Voekl, E.; Piston, D.; Gauvin, R.; Lockley, A. J.; Bailey, G. W., and McKernan, S., Eds.; Cambridge University Press, New York, NY, 2002, p. 832CD.
品名 |
产品描述 |
货号 |
规格 |
GoldiBlot™ HIS Western Blot Kit |
用于在蛋白杂交中检测his重组蛋白的 |
2090 |
15个杂交 |
References:
品名 |
产品描述 |
货号 |
规格 |
1-Mercapto-(triethylene glycol) methyl ether functionalized gold nanoparticles |
两性3 - 5 nm纳米金颗粒,溶于甲苯、氯仿、乙酸乙酯、丙酮、水、乙醇等溶剂 |
3012 |
80 mg |
(1-Mercaptoundec-11-yl) tetraethyleneglycol functionalized gold nanoparticles |
亲水3 - 5 nm纳米金颗粒,溶于乙醇、水 |
3013 |
80 mg |
Dodecanethiol functionalized gold nanoparticles |
疏水3 - 5 nm纳米金,用于甲苯等有机溶剂 |
3014 |
80 mg |
Octanethiol functionalized gold nanoparticles |
疏水2 - 4 nm纳米金,用于甲苯等有机溶剂 |
3015 |
80 mg |
AuroVistTM :X-射线造影剂
Live mouse 1 hour after injection with AuroVist™, showing kidney contrast and fine structure (bar = 1 mm).
(upper): Live mouse, 2 minutes after injection
showing vascular fine structure; (lower) MicroCT of
mouse inferior vena cava (bar = 1mm).
品名 |
产品描述 |
货号 |
规格 |
AuroVistTM-1.9nm |
在水、PBS或其他缓冲液中即溶 |
1102 |
40 mg Au |
AuroVistTM-15nm |
产品已溶于PBS,并经0.22 um 膜过滤 |
1115 |
40 mg Au |
品名 |
产品描述 |
货号 |
规格 |
Nanogold-链酶亲和素 |
|
2016 |
0.5ml |
1ml |
|||
Nanogold-山羊抗生物素 |
IgG |
2015 |
0.5ml |
1ml |
|||
Nanogold-山羊抗小鼠IgG |
IgG |
2001 |
0.5ml |
1ml |
|||
Fab’ |
2002 |
0.5ml |
|
1ml |
|||
Nanogold-山羊抗兔IgG |
IgG |
2003 |
0.5ml |
1ml |
|||
Fab’ |
2004 |
0.5ml |
|
1ml |
|||
Nanogold-兔抗山羊IgG |
IgG |
2005 |
0.5ml |
1ml |
|||
Fab’ |
2006 |
0.5ml |
|
1ml |
|||
Nanogold-山羊抗大鼠IgG |
IgG |
2007 |
0.5ml |
1ml |
|||
Fab’ |
2008 |
0.5ml |
|
1ml |
|||
Nanogold-兔抗绵羊IgG |
IgG |
2050 |
0.5ml |
1ml |
|||
Fab’ |
2051 |
0.5ml |
|
1ml |
|||
Nanogold-山羊抗人IgG |
IgG |
2052 |
0.5ml |
1ml |
|||
Fab’ |
2053 |
0.5ml |
|
1ml |
|||
Nanogold-山羊抗豚鼠IgG |
IgG |
2054 |
0.5ml |
1ml |
|||
Fab’ |
2055 |
0.5ml |
|
1ml |
用CARD扩增的纳米金+银增强检测Hela细胞中单个拷贝HPV16的原位杂交
扫描透射电镜图片 (左)羊抗小鼠胶体金 (右)羊Fab’抗小鼠纳米金
References:
Nanogold® Antibody Conjugates
品名 |
产品描述 |
货号 |
规格 |
兔抗山羊IgG Alexa Fluor® 488 荧光纳米金 |
1.4nm, 连接了Alexa Fluor® 488的亲和纯化的Fab’ |
7206 |
0.5ml |
1ml |
|||
山羊抗豚鼠IgG Alexa Fluor® 488 荧光纳米金 |
1.4nm, 连接了Alexa Fluor® 488的亲和纯化的Fab’ |
7255 |
0.5ml |
1ml |
|||
山羊抗人IgG Alexa Fluor® 488 荧光纳米金 |
1.4nm, 连接了Alexa Fluor® 488的亲和纯化的Fab’ |
7253 |
0.5ml |
1ml |
|||
山羊抗小鼠IgG Alexa Fluor® 488 荧光纳米金 |
1.4nm, 连接了Alexa Fluor® 488的亲和纯化的Fab’ |
7202 |
0.5ml |
1ml |
|||
山羊抗兔IgG Alexa Fluor® 488 荧光纳米金 |
1.4nm, 连接了Alexa Fluor® 488的亲和纯化的Fab’ |
7204 |
0.5ml |
1ml |
|||
山羊抗大鼠IgG Alexa Fluor® 488 荧光纳米金 |
1.4nm, 连接了Alexa Fluor® 488的亲和纯化的Fab’ |
7208 |
0.5ml |
1ml |
|||
兔抗绵羊IgG Alexa Fluor® 488 荧光纳米金 |
1.4nm, 连接了Alexa Fluor® 488的亲和纯化的Fab’ |
7251 |
0.5ml |
1ml |
|||
链酶亲和素Alexa Fluor® 488 荧光纳米金 |
1.4nm, 连接了Alexa Fluor® 488的亲和纯化的Fab’ |
7216 |
0.5ml |
1ml |
Alexa Fluor®* 546荧光纳米金结合物:
品名 | 产品描述 |
货号 |
规格 |
山羊抗豚鼠IgG Alexa Fluor®* 546荧光纳米金 |
1.4nm, 连接了Alexa Fluor® 546的亲和纯化的Fab’ |
7455 |
0.5ml |
1ml |
|||
山羊抗小鼠IgG Alexa Fluor®* 546荧光纳米金 |
1.4nm, 连接了Alexa Fluor® 546的亲和纯化的Fab’ |
7402 |
0.5ml |
1ml |
|||
山羊抗兔IgG Alexa Fluor®* 546荧光纳米金 |
1.4nm, 连接了Alexa Fluor® 546的亲和纯化的Fab’ |
7404 |
0.5ml |
1ml |
|||
链酶亲和素Alexa Fluor®* 546荧光纳米金 |
1.4nm, 连接了Alexa Fluor® 546的亲和纯化的Fab’ |
7416 |
0.5ml |
1ml |
Alexa Fluor® 594荧光纳米金结合物:
品名 | 产品描述 |
货号 |
规格 |
山羊抗豚鼠IgG Alexa Fluor® 594荧光纳米金 |
1.4nm, 连接了结合Alexa Fluor® 594的亲和纯化的Fab’ |
7355 |
0.5ml |
1ml |
|||
山羊抗小鼠IgG Alexa Fluor® 594荧光纳米金 |
1.4nm, 连接了结合Alexa Fluor® 594的亲和纯化的Fab’ |
7302 |
0.5ml |
1ml |
|||
山羊抗兔IgG Alexa Fluor® 594荧光纳米金 |
1.4nm, 连接了结合Alexa Fluor® 594的亲和纯化的Fab’ |
7304 |
0.5ml |
1ml |
|||
链酶亲和素Alexa Fluor®* 594荧光纳米金 |
1.4nm, 连接了结合Alexa Fluor® 594的亲和纯化的Fab’ |
7316 |
0.5ml |
1ml |
References:
Fluorescein FluoroNanogold Conjugates
Fluorescein FluoroNanogold Conjugates
A prototype was used in the following publication:
Huang, S.; Deerinck, T. J.; Ellisman, M. H., and Spector, D. L.: In vivo analysis of the stability and transport of nuclear poly(A)+ RNA; J. Cell. Biol., 126, 877-899 (1994).
Cy3®-FluoroNanogold Conjugates
品名 |
产品描述 |
货号 |
规格 |
Palmitoyl Nanogold® |
共价结合C15棕榈酸的1.4nm纳米金颗粒 |
4020 |
30 nmol |
DPPE Nanogold® |
共价结合二棕榈酰磷酯酰乙醇胺的1.4nm纳米金颗粒 |
4021 |
30 nmol |
Palmitoyl Undecagold |
共价结合C15棕榈酸的0.8nm纳米金颗粒 |
4022 |
30 nmol |
DPPE Undecagold |
共价结合二棕榈酰磷酯酰乙醇胺的0.8nm纳米金颗粒 |
4023 |
30 nmol |
References:
品名 |
产品描述 |
货号 |
规格 |
Monomaleimido Nanogold®(MMN) |
用于标记巯基,可标记Fab’、抗体、含半胱氨酸的蛋白质或其他含氢巯基的复合物, 1.4 nm |
2020 |
30 nmol |
Monoamino Nanogold® |
1.4nm, 标记糖蛋白的碳水化合物部分或其他应用。 |
2021 |
30 nmol |
Mono-Sulfo-NHS-Nanogold® |
14nm,标记伯胺 |
2025 |
30 nmol |
Positively Charged Nanogold® |
1.4nm,带正电,含多个胺,用于结合带负电的位点,或其他偶联方案。 |
2022 |
30 nmol |
Negatively Charged Nanogold® |
1.4nm,带负电,含多个胺,用于结合带正电的位点,或其他偶联方案。 |
2023 |
30 nmol |
Nanogold® Particles, Non- Functionalized |
1.4nm金颗粒,冻干粉,非活性形式 |
2010 |
30 nmol |
Positively Charged Nanogold®
Recent References: Monomaleimido-Nanogold®
Other Nanogold® Reagents
Nanogold® Nitrilotriacetic acid-Ni(II)
品名 |
货号 |
规格 |
Monomaleimido Undecagold |
2030 |
50 nmol |
Monoamino Undecagold |
2031 |
50 nmol |
Mono-Sulfo-NHS-Undecagold |
2045 |
50 nmol |
Positively Charged Undecagold |
2043 |
50 nmol |
Negatively Charged Undecagold |
2044 |
50 nmol |
Undecagold Particles, Non- Functionalized |
2060 |
50 nmoles |
References:
Undecagold: Recent New References
品名 |
产品描述 |
货号 |
规格 |
GoldEnhance LM/Blot (GELM) |
金增强试剂,用于光镜样品,4种溶液,使用前混合 |
2112 |
Initiator/Moderator/Activator/Buffer |
GoldEnhance EM(GEEM) |
金增强试剂,用于电镜样品,4种溶液,使用前混合 |
2113 |
Initiator/Moderator/Activator/Buffer |
HQ Silver |
用于纳米金的质量最佳的银增强试剂,均匀显影,极好的保持结构,特别适合电镜,光敏感 |
2012 |
Initiator/Moderator/Activator |
Li Silver |
纳米金的银增强,用于电镜、光镜、凝胶、杂交,光不敏感 |
2013 |
Initiator/Enhancer |
HQ and LI Silver
品名 |
产品描述 |
货号 |
规格 |
NanoVan® (Methylamine Vanadate) |
2%钒负染染料 |
2011 |
5 ml |
Nano-W® (Methylamine Tungstate) |
2%基于有机钨复合物的负染染料 |
2018 |
5 ml |
References:
NanoVan Negative Stain
Nano-W Negative Stain
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