Actin RNA Probe, DIG-labeled

Actin RNA Probe, DIG-labeled

solution, pkg of 2 μg

一般描述

This antisense RNA probe (human β-actin) is in vitro transcribed in the presence of digoxigenin (DIG)-UTP. The transcript has a length of 588 bases. 550 bases are complementary to the 5′ region of human β-actin mRNA (nucleotides 69 to 6l8, EMBL: HSAC07). The additional 25 bases at the 5′ end, and the 13 additional bases at the 3′ end of the transcript are specific for the promoter/polylinker region of the transcription vector.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

应用

Actin RNA Probe, DIG-labeled, is specifically useful for evaluating the quality and quantity of various RNA species and can be used:

• in In situ hybridization (for example, as a control in mRNA detection)
• for quality control in the construction of cDNA libraries
• in northern blot analysis to evaluate RNA from various human cell lines and tissue samples

组分

Actin RNA Probe, DIG-labeled, is supplied in autoclaved, DEPC-treated water.

序列

Agarose gel electrophoresis under denaturing conditions and subsequent northern blot analysis reveal a defined band of 588 bases.