- 询价
- roche
- 罗氏11498045910
- 2025年08月04日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
嵘崴达
- 英文名：
Actin RNA Probe, DIG-labeled
- 库存：
需确认
- 保质期：
见产品外包装
- 保存条件：
−20°C
- 规格：
2 μG
Actin RNA Probe, DIG-labeled
solution, pkg of 2 μg
一般描述
This antisense RNA probe (human β-actin) is in vitro transcribed in the presence of digoxigenin (DIG)-UTP. The transcript has a length of 588 bases. 550 bases are complementary to the 5′ region of human β-actin mRNA (nucleotides 69 to 6l8, EMBL: HSAC07). The additional 25 bases at the 5′ end, and the 13 additional bases at the 3′ end of the transcript are specific for the promoter/polylinker region of the transcription vector.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
应用
Actin RNA Probe, DIG-labeled, is specifically useful for evaluating the quality and quantity of various RNA species and can be used:
- in In situ hybridization (for example, as a control in mRNA detection)
- for quality control in the construction of cDNA libraries
- in northern blot analysis to evaluate RNA from various human cell lines and tissue samples
组分
Actin RNA Probe, DIG-labeled, is supplied in autoclaved, DEPC-treated water.
序列
Agarose gel electrophoresis under denaturing conditions and subsequent northern blot analysis reveal a defined band of 588 bases.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验DIG (Digoxigenin) labeled RNA probe In situ hybridization protocol
tubes. Heat for 95°C for 2 minutes on a PCR block. This will dehybridize the RNA or DNA probe. Chill on ice immediately to prevent rehybridization. 9. Drain off the hybridization solution. Add 50 to 100 ul of diluted probe per section (ensure
Preparation and purification of biotin-labeled probe for ISH
Preparation and purification of biotin-labeled probe for ISH I. Biotin-labeling of plasmid or genomic DNA. Suggested chemicals: Nick Translation Kit, Enzo Diagnostics Cat. No. 42803. Prepare the following
I采用了显色法，使用显色底物 NBT/BCIP；而Starter Kit II选用化学发光法底物CSPD。 如果您用来合成探针的DNA模板的量比较少或是质量不够高的时候，建议您使用PCR DIG Probe Synthesis Kit。该试剂盒专用于制备高灵敏度的杂交探针，例如单拷贝的基因。其另一个特点就是实验中无需定量斑点检测过程，通过定量凝胶电泳就可以快速确定PCR标记探针的效率。
技术资料暂无技术资料 索取技术资料
