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- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
−20°C
- 供应商：
嵘崴达
- 英文名：
DNA Molecular Weight Marker XVI (250 bp ladder)
- 库存：
官网显示已停产
- 保质期：
见产品外包装
- 规格：
50 μG
pkg of 50 μg (in 200 μl), solution
别名:DNA marker
一般描述
Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases.Size Range: 0.25 to 3.09 kbp
应用
DNA Molecular Weight Marker XVI (250 bp ladder) may be used as a molecular weight marker in agarose gel electrophoresis:- for the molecular weight comparison of random amplified polymorphic DNA (RAPD) amplified fragments of plant genomic DNA and meat products
- for enterobacterial repetitive intergenic consensus (ERIC) products generated from polymerase chain reaction
- in reverse transcription-polymerase chain reaction (RT-PCR) amplified dsRNA sequences of African horse sickness virus
序列
The mixture contains 12 blunt-ended, double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 250, 500, 750, 1000, 1250, 1500, 1750, 2000, 2250, 2500, 2750, and 3000. The 1000- and 2000-bp banding pattern are 3 times brighter. Electrophoretic separation of this molecular weight marker results in a regular pattern.Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. The fragments exhibit uniform spacing (e.g., on a 0.7% agarose gel) for easier reading, while bands 1000 and 2000bp are 3 times brighter for easy orientation.
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文献和实验Molecular Weight Marker l Hin dIII Digest Marker Solution Digest 20 µg l DNA (40 µl DNA if at 0.5 µg/µl) Add 50 µl 10X Tracking Dye Bring volume of the digested DNA up to 500 µl
例一 gongyulai ：我用的是药物诱导癌细胞凋亡，做DNA Ladder（用的是北京鼎国的动物细胞凋亡梯子提取试剂盒）。跑出来的电泳不是一条条带，而是每条很长的拖尾现象，Marker到是出来了。我是按说明书上0。8%的琼脂糖。我用的是60v电压。不知道什么原因？郁闷。说明书上说混合温和是什么意思，我又没用力摇。我还想问收集凋亡细胞时离心时转速多少比较好？chujun_hust ：（1）“跑出来的电泳不是一条条带，而是每条很长的拖尾现象”： 可能是由于你点样时，时间太长了，导致样品扩散
The Isolation of High Molecular Weight Eukaryotic DNA
The isolation of high molecular weight eukaryotic DNA in good yield is an important prerequisite for the analysis of specific sequences by Southern blotting (Chapter 9 ), or for molecular cloning in phage or cosmid vectors (Chapter 49 ).
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