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发货周期:现货
博士德生物
cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Mouse
96T
货号 | EK0351 |
---|---|
产品名称 | Mouse Fibronectin(FN1) ELISA Kit |
产品描述 | For quantitative detection of mouse Fibronectin in cell culture supernates, serum and plasma(heparin, EDTA, citrate). |
免疫原 | from plasma |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <15pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | FN1 |
基因全名 | fibronectin 1 |
Uniprot ID | FN1: P11276 |
指标别名 | CIG|Cold insoluble globulin|Cold-insoluble globulin|Fibronectin 1|FINC|FINC_HUMAN|FN|FN1|FNZ|Migration stimulating factor|MSF|Ugl-Y3 |
背景资料 | Fibronectin(FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer. |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0351-CAP | 96-well plate precoated with anti- mouse Fibronectin antibody | 1 |
EK0351-ST | Lyophilized mouse Fibronectin standard | 10ng/tube×2 |
EK0351-DA | Biotinylated anti- mouse Fibronectin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 206 | 1683 | 4323 | 219 | 1843 | 3947 |
Standard deviation | 13.39 | 126.22 | 293.96 | 15.54 | 160.34 | 335.49 |
CV(%) | 6.5% | 7.5% | 6.8% | 7.1% | 8.7% | 8.5% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.066 | 0.140 | 0.188 | 0.320 | 0.512 | 0.879 | 1.561 | 2.175 |
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