中文名称 |
血管内皮细胞迁移蛋白抗体 |
别 名 |
angio-associated migratory cell protein; AAMP_HUMAN. |
规格价格 |
100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 |
100ul 200ul |
研究领域 |
肿瘤 心血管 细胞生物 细胞粘附分子 血管内皮细胞 |
抗体来源 |
Rabbit |
克隆类型 |
Polyclonal |
交叉反应 |
Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, |
产品应用 |
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 |
47kDa |
细胞定位 |
细胞浆 细胞膜 |
性 状 |
Lyophilized or Liquid |
浓 度 |
1mg/ml |
免 疫 原 |
KLH conjugated synthetic peptide derived from human AAMP (76-125aa):76-125/434 |
亚 型 |
IgG |
纯化方法 |
affinity purified by Protein A |
储 存 液 |
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed |
PubMed |
产品介绍 |
background: AAMP is a 434 amino acid immunoglobulin-like protein that contains 8 WD repeats. Expressed in endothelial cells, cytotrophoblasts and blood vessels, AAMP is thought to have a heparin-sensitive role in cell adhesion and cell migration. AAMP is strongly expressed in poorly differentiated colon adenocarcinoma cells, suggesting a role for AAMP in tumor progression. Function: The gene product of AAMP is an immunoglobulin-type protein, which is found to be expressed strongly in endothelial cells, cytotrophoblasts, and poorly differentiated colon adenocarcinoma cells found in lymphatics. The protein contains a heparin-binding domain and mediates heparin-sensitive cell adhesion. Subcellular Location: Cell Membrane and Cytoplasmic Tissue Specificity: Expressed in metastatic melanoma, liver, skin, kidney, heart, lung, lymph node, skeletal muscle and brain, and also in A2058 melanoma cells and activated T-cells (at protein level). Expressed in blood vessels. Strongly expressed in endothelial cells, cytotrophoblasts, and poorly differentiated. colon adenocarcinoma cells found in lymphatics. Similarity: Contains 8 WD repeats. SWISS: Q13685 Gene ID: 14 Database links: Entrez Gene: 14 Human Entrez Gene: 227290 Mouse Entrez Gene: 301512 Rat Omim: 603488 Human SwissProt: Q3SZK1 Cow SwissProt: Q13685 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 |
Blank control(blue): Raji(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-AAMP antibody(bs-11388R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |