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发货周期:现货
博士德生物
cell culture supernates, serum and plasma(heparin, EDTA, citrate).
96T
货号 | EK0337 |
---|---|
产品名称 | Human FASL ELISA Kit |
产品描述 | For quantitative detection of human FASL in cell culture supernates, serum and plasma(heparin, EDTA, citrate). |
免疫原 | CHO, P134-L281 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <2pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | FASLG |
基因全名 | Fas ligand |
Uniprot ID | P48023 |
指标别名 | Apoptosis (APO 1) antigen ligand 1|Apoptosis antigen ligand|APT1LG1|APTL|CD178|CD95 L|CD95 ligand|CD95L|CD95L protein|Fas antigen ligand|FASL|FASLG|TNF superfamily member 6|TNFSF6|Tumor necrosis factor (ligand) superfamily member 6|Tumor necrosis factor ligand superfamily member 6 |
背景资料 | Fas ligand(FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor(TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0337-CAP | 96-well plate precoated with anti- human FASL antibody | 1 |
EK0337-ST | Lyophilized recombinant human FASL standard | 10ng/tube×2 |
EK0337-DA | Biotinylated anti- human FASL antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 36 | 186 | 437 | 34 | 189 | 419 |
Standard deviation | 2.19 | 9.67 | 28.84 | 2.1 | 12.47 | 33.1 |
CV(%) | 6.1% | 5.2% | 6.6% | 6.2% | 6.6% | 7.9% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.008 | 0.114 | 0.196 | 0.379 | 0.740 | 1.371 | 2.121 | 2.693 |
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