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NSE antibody

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  • ¥1380 - 2200
  • 康朗生物
  • 中国/美国/德国
  • 2026年03月02日
  • WB=1:500-2000
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商

      上海康朗生物科技有限公司

    • 库存

      大量

    • 目录编号

      kl-10829R

    • 克隆性

      多克隆

    • 抗原来源

      Rabbit

    • 保质期

      12个月

    • 抗体英文名

      NSE antibody

    • 抗体名

      神经元特异性烯醇化酶/γ 烯醇化酶抗体

    • 宿主

      Rabbit

    • 适应物种

      Human

    • 免疫原

      Recombinant human NSE protein:

    • 亚型

      IgG

    • 形态

      冻干粉或液体

    • 应用范围

      WB=1:500-2000

    • 浓度

      1mg/ml

    • 保存条件

      -20 °C

    • 规格

      100ul 200ul

    NSE antibody
    中文名称 神经元特异性烯醇化酶/γ 烯醇化酶抗体
    别    名 Gamma-enolase; Gamma enolase; Neural enolase; Neuron specific enolase; neurone-specific enolase; 2 phospho D glycerate hydrolyase; Eno 2; Eno2; ENO2; ENOG; Enolase 2; Enolase 2 gamma neuronal; Enolase2; Neuron specific enolase; Neuron specific gamma enolase; NSE; ENOG_HUMAN; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; enolase 2, gamma, neuronal.  
    规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 100ul  200ul
    研究领域 肿瘤  细胞生物  免疫学  神经生物学  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, 
    产品应用 WB=1:500-2000 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 48kDa
    细胞定位 细胞浆 细胞膜 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 Recombinant human NSE protein: 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008].

    Function:
    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

    Subunit:
    Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.

    Subcellular Location:
    Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.

    Tissue Specificity:
    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

    Similarity:
    Belongs to the enolase family.

    SWISS:
    P09104

    Gene ID:
    2026

    Database links:

    Entrez Gene: 2026 Human

    Entrez Gene: 13807 Mouse

    Entrez Gene: 24334 Rat

    Omim: 131360 Human

    SwissProt: P09104 Human

    SwissProt: P17183 Mouse

    SwissProt: P07323 Rat

    Unigene: 511915 Human



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

    神经元特异性烯醇化酶(NSE)存在于神经元细胞和神经内分泌组织中,起源于神经内分泌细胞的肿瘤可产生过量的NSE。NSE也是小细胞肺癌的检测指标,70%左右的小细胞肺癌患者血中NSE升高,而其他组织型肺癌NSE升高的患者仅为10%~20%。
    产品图片 产品细节图片1
    Sample: 
    U-251 MG (Human) Lysate at 30 ug
    Primary: Anti- NSE (bs-10829R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 48 kD
    Observed band size: 48 kD

    产品细节图片2
    Sample: 
    U251(Human) cellLysate at 40 ug
    U-87MG(Human) cell Lysate at 40 ug
    Primary: Anti-NSE(bs-10829R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 48 kD
    Observed band size: 48 kD

    产品细节图片3
    Sample: 
    U-87 MG (Human) Lysate at 30 ug
    Primary: Anti- NSE (bs-10829R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 48 kD
    Observed band size: 48 kD

    产品细节图片4
    Sample: 
    Hep G2 (Human) Lysate at 30 ug
    Primary: Anti- NSE (bs-10829R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 48 kD
    Observed band size: 48 kD

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    图标文献和实验
    相关实验
    • Generation of Antibody Molecules Through Antibody Engineering

      been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional

    • The Antibody Molecule

      The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera

    • Antibody Storage

        General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease

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