RANKL/CD254 antibody

价格¥780 - 2200

品牌康朗生物
货号kl-0747R
产地中国/美国/德国
更新日期2025年07月16日

应用范围WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500
宿主Rabbit
适应物种Human, Mouse, Rat, Dog,

上海康朗生物科技有限公司

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技术资料

供应商：
上海康朗生物科技有限公司
库存：
大量
目录编号：
kl-0747R
克隆性：
多克隆
抗原来源：
Rabbit
保质期：
12个月
抗体英文名：
RANKL/CD254 antibody
抗体名：
骨保护蛋白配体/破骨细胞分化因子抗体
宿主：
Rabbit
适应物种：
Human, Mouse, Rat, Dog,
免疫原：
KLH conjugated synthetic peptide derived from human OPGL:210-317/317
亚型：
IgG
形态：
冻干粉或液体
应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500
浓度：
1mg/ml
保存条件：
-20 °C
规格：
50ul 100ul 200ul

RANKL/CD254 antibody

中文名称	骨保护蛋白配体/破骨细胞分化因子抗体
别名	OPGL; CD254; hRANKL2; ODF; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; RANKL; Receptor activator of nuclear factor kappa B ligand; sOdf; SOFA; TNF related activation induced cytokine; TNFSF 11; TNFSF11; TRANCE; Tumor necrosis factor ligand superfamily member 11; Osteoprotegerin Ligand; TNF11_HUMAN.
	Specific References (15) \| bs-0747R has been referenced in 15 publications. [IF=1.77] Li, Ping. "Efficacy and Safety of Echinacoside in a Rat Osteopenia Model." Evidence-Based Complementary and Alternative Medicine 2013 (2013). Rat. PubMed:23573159 [IF=1.68] Sun, Jiabing, et al. "A Crucial Role of IL-17 in Bone Resorption During Rejection of Fresh Bone Xenotransplantation in Rats." Cell Biochemistry and Biophysics: 1-7. IHC-P ; Rat. PubMed:25331672 [IF=1.98] Yu, Xijiao, et al. "Expression of neuropeptides and bone remodeling-related factors during periodontal tissue regeneration in denervated rats." Journal of Molecular Histology: 1-9. WB ; Rat. PubMed:25663522 [IF=2.38] Zhu, Wenjing, et al. "Effect of PI3K/Akt Signaling Pathway on the Process of Prostate Cancer Metastasis to Bone." Cell Biochemistry and Biophysics (2015): 1-7. WB ; Human. PubMed:22870197 [IF=2.84] Ota, Takehiro, et al. "Expression of colony-stimulating factor 1 is associated with occurrence of osteochondral change in pigmented villonodular synovitis."Tumor Biology (2015): 1-7. IHC-P ; Human. PubMed:25854167 [IF=1.88] Li, Xianxian, et al. "Oral administration of 5-Hydroxytryptophan aggravated periodontitis-induced alveolar bone loss in rats." Archives of Oral Biology(2015). IHC-P ; Rat. PubMed:25766472 [IF=0.94] Zhang, Yuanyu, et al. "Mycobacterium tuberculosis 10-kDa co-chaperonin regulates the expression levels of receptor activator of nuclear factor-κB ligand and osteoprotegerin in human osteoblasts." Experimental and Therapeutic Medicine. WB ; Human. PubMed:25667654 [IF=2.09] Chen, Zhiguang, et al. "Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway."International Journal of Molecular Medicine. WB ; Rat. PubMed:26677102 [IF=2.68] Xiao, Wanan, et al. "Bone fracture healing is delayed in splenectomic rats." Life Sciences (2016). WB ; Rat. PubMed:27956350 [IF=2.62] Zhang, Xiaonan, et al. "Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway." Chemico-Biological Interactions 256 (2016): 188-197. WB ; Rat. PubMed:27387537 [IF=1.69] Dolci, Gabriel Schmidt, et al. "Atorvastatin-induced osteoclast inhibition reduces orthodontic relapse." American Journal of Orthodontics and Dentofacial Orthopedics 151.3 (2017): 528-538. IHC-P ; Rat. PubMed:28257738 [IF=1.56] He, Ming, et al. "Effect of glucocorticoids on osteoclast function in a mouse model of bone necrosis." Molecular medicine reports 14.2 (2016): 1054-1060. Mouse. PubMed:27277157 [IF=2.66] Yu, X., et al. "Denervation effectively aggravates rat experimental periodontitis." Journal of Periodontal Research (2017). WB ; Rat. PubMed:28621056 [IF=3.08] Zhan, Fu‐Liang, Xin‐Yang Liu, and Xing‐Bo Wang. "The Role of MicroRNA‐143‐5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway." Journal of Cellular Biochemistry (2017). WB ; Human. PubMed:28608628 [IF=1.75] Chen, Helin, et al. "Intermittent administration of parathyroid hormone ameliorated alveolar bone loss in experimental periodontitis in streptozotocin-induced diabetic rats." Archives of Oral Biology (2017). IHC-P ; Rat. PubMed:28732226
规格价格	50ul/780元购买 100ul/1380元购买 200ul/2200元购买大包装/询价
说明书	50ul 100ul 200ul
研究领域	肿瘤细胞生物免疫学发育生物学
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Dog,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	35kDa
细胞定位	细胞浆细胞膜分泌型蛋白
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human OPGL:210-317/317
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008]. Function: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Subcellular Location: Cytoplasm; Secreted and Cell membrane. Tissue Specificity: Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. Post-translational modifications: The soluble form of isoform 1 derives from the membrane form by proteolytic processing. The cleavage may be catalyzed by ADAM17. DISEASE: Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. Similarity: Belongs to the tumor necrosis factor family. SWISS: O14788 Gene ID: 8600 Database links: Entrez Gene: 8600 Human Entrez Gene: 21943 Mouse Omim: 602642 Human SwissProt: O14788 Human SwissProt: O35235 Mouse Unigene: 333791 Human Unigene: 249221 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. OPGL骨保护蛋白配体又称骨保护素配体(破骨细胞发育刺激因子)。属肿瘤坏死因子TNF-a家族。 OPGL促进破骨细胞的分化和活性,而OPG抑制这些过程。骨髓瘤细胞影响骨髓中这两种蛋白的生理平衡，是发生溶骨性病变的根本所在。
产品图片	Sample: HUVEC-C Cell (Human) Lysate at 40 ug HepG2 Cell (Human) Lysate at 40 ug Primary: Anti-RANKL/CD254 (bs-0747R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35kD Observed band size: 50kD Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-OPGL/RANKL/ODF Polyclonal Antibody, Unconjugated(bs-0747R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-OPGL/RANKL/ODF Polyclonal Antibody, Unconjugated(bs-0747R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: mouse splenocytes(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).