WB IF IP (Optimal dilutions should be determined by the end user)
Notes
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein.
Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Purity
Immunogen affinity purified
Clonality
Monoclonal
Isotype
IgG
Alternative names
GKPIPNPLLGLDST tag antibody, rev epitope tag antibody
Western Blot: 1ug V5 fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000
IF analysis of 293 cells transfected with a V5-tag protein, using TDYbio anti-V5Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-V5)
IP antibody use:5ug V5 Mouse IgG1 per ml Lysate,WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with V5-tag fusion protein 3、IP(untransfted 293+anti-V5 mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5、IP (transfected 293+anti-V5 mAb+ Protein G agarose) 6、IP (transfected 293+Protein G) 7、Recombinant protein (E.coli)