Recombinant eGFP (Recombinant Enhanced Green Fluorescent Protein; reGFP);重组增强绿色荧光蛋白
Synonyms
eGFP; GFPuv; Enhanced Green Fluorescent Protein
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
N/A
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
Endotoxin
Less than 1EU/μg of reGFP as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Others
Background
Green fluorescent protein (GFP) here refers to the protein first purified from jellyfish Aequorea victoria, though many other organisms have similar proteins. It is a 26.9kDa protein (composed of 238a.a. residues) that shows green fluorescence in shortwave light (blue to ultraviolet). Despite of wild-type GFP, many mutants of GFP have been engineered for wider usage in research. Enhanced GFP (eGFP) has S65T and F64L mutations, which make GFP show increased fluorescence and fold more efficiently under 37℃, respectively. eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in fluids.