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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Recombinant Murine DHH C23II
- 保质期:
一年
- 供应商:
上海联迈生物工程有限公司
- 保存条件:
-20℃或更低温度保存
- 规格:
5μg
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| LM7338-5μg | Recombinant Murine DHH C23II | 5μg | 464.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Mouse | 13363 | LM61488 | E. coli | 177aa | 20kDa | - |
| About this Antibody | |
| Name | Recombinant Murine DHH C23II (Recombinant Murine Desert Hedgehog Cys23IleIle; rMuDHH C23II); Recombinant Mouse DHH C23II ; 重组小鼠沙漠刺猬因子C23II |
| Synonyms | DHH; desert hedgehog; desert hedgehog (Drosophila) homolog; desert hedgehog homolog; desert hedgehog protein; HHG-3MGC35145 |
| Purity | >98% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by murine MC3T3-E1 cells is 5-20μg/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, 1mM DTT, 0.05% Tween-80. |
| Endotoxin | Less than 0.1EU/μg of rMuDHH C23II as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Others |
| Background | Desert hedgehog protein also known as HHG-3 is a member of the hedgehog family which is encoded by the DHH gene. It is expressed in adult testes but not expressed in limb buds. DHH may be as a precursor that is auto-catalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. Besides, DHH may have a function as a spermatocyte survival factor in the testes. Within the N-terminal peptide, murine DHH shares 97% and 100% amino acid (a.a.) sequence identity with human and rat DHH, respectively. |
| Amino Acid Sequence | IIGPGRGPVG RRRYVRKQLV PLLYKQFVPS MPERTLGASG PAEGRVTRGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH IHVSVKADNS LAVRAGG |
| 产品编号 | 产品名称 | 包装 |
| LM7338-5μg | Recombinant Murine DHH C23II | 5μg |
| LM7338-25μg | Recombinant Murine DHH C23II | 25μg |
| LM7338-100μg | Recombinant Murine DHH C23II | 100μg |
| LM7338-1mg | Recombinant Murine DHH C23II | 1mg |
| - | 说明书 | 1份 |
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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