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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 英文名：
EagleTaq Universal Master Mix (ROX)
- 供应商：
上海善然生物科技有限公司
- 保存条件：
低温
产品描述
Components
Contains EagleTaq DNA Polymerase and a reaction buffer with dATP, dCTP, dGTP, dUTP, MgCl2, and ROX.
General description
2× concentrated, ready-to-use hot start master mix for qPCR and qRT-PCR using the hydrolysis probe detection format on real-time PCR instruments.
The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated PCR master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR hydrolysis probe reactions. It contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
The EagleTaq Universal Master Mix (ROX) contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
The hot start properties allow reaction setup at ambient temperature.
With this robust reagent, any PCR protocol optimization is minimized
EagleTaq Universal Master Mix (ROX); Instructions For Use
Components
Contains EagleTaq DNA Polymerase and a reaction buffer with dATP, dCTP, dGTP, dUTP, MgCl2, and ROX.
General description
2× concentrated, ready-to-use hot start master mix for qPCR and qRT-PCR using the hydrolysis probe detection format on real-time PCR instruments.
The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated PCR master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR hydrolysis probe reactions. It contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
The EagleTaq Universal Master Mix (ROX) contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
The hot start properties allow reaction setup at ambient temperature.
With this robust reagent, any PCR protocol optimization is minimized
EagleTaq Universal Master Mix (ROX); Instructions For Use
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文献和实验相关实验
Taq Talk 荧光定量 PCR 系列课程之【qPCR 预混液的选择】一起来了解如何高效地选择合适的 qPCR 预混液
相关专题 随着定量PCR 在不断改善，多重qPCR同样如此。多重qPCR是将几个PCR分析合在一起，同时扩增并独立检测目标，它在提高通量的同时也节省了样品。与单重qPCR相比，尽管它需要更多时间和精力，但用户得到了丰富的数据。 然而，多重qPCR 并不是单重反应的简单叠加，因为在一个反应管中，任何一个目标的扩增都能影响其他目标的扩增。让反应条件同时适合这么多个目标，这似乎不太容易，但使用qPCR预混液可能有帮助。预混
扩增曲线的分析攻略。 1、确认软件设置是否正确 对照试剂盒说明书，检查时间、温度、循环数、荧光采集等是否设置正确。有一个比较容易忽略的设置问题是，需要看下所选用的试剂中是否含有 ROX 来作为参比荧光染料。Applied Biosystems™ 系列荧光定量 PCR 仪器可以用 ROX 来作为参比荧光染料，用以校正仪器系统以外的物理误差，比如均一化校正由于移液误差或者蒸发导致的批内体积误差等。目前市面上有的的荧光定量 PCR 预混液是不含有 ROX 的，当用此类试剂的时候，应该将 ROX 参比
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EagleTaq PCR预混液
¥1538.01
