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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Recombinant SK
- 保质期:
一年
- 供应商:
上海联迈生物工程有限公司
- 保存条件:
-20℃或更低温度保存
- 规格:
1mg
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| LM7046-1mg | Recombinant SK | 1mg | 2338.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| - | 13902388 | LM4Q6S2 | E. coli | 414aa | 47.3kDa | - |
| About this Protein | |
| Name | Recombinant SK (Recombinant Streptokinase; rSK);重组链激酶 |
| Synonyms | Streptokinase; ska; SDSE_0239 |
| Purity | >97% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The specific activity determined by fibrining lysis in agarose plate is 8.0×104IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin | Less than 1EU/μg of rSK as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Enzymes |
| Background | Streptokinase is a thrombolytic agent i.e. it dissolves vascular thrombi. This potent agent is derived from the beta haemolytic streptococci and is consequently associated with the risk of anaphylaxis. Streptokinase acts by stimulating the conversion of plasminogen to plasmin. It's a proteolytic enzyme which is able to disrupt fibrin stability and production. The half-life of streptokinase is 6 hours. |
| Amino Acid Sequence | IAGPEWLLDR PSVNNSQLVV SVAGTVEGTN QDISLKFFEI DLTSRPAHGG KTEQGLSPKS KLFATDSGAM PHKLEKADLL KAIQEQLIAN VHSNDDYFEV IDFASDATIT DRNGKVYFAD KDGSVTLPIQ PVQEFLLKGH VRVRPYKEKP VQNQAKSVDV EYTVQFTPLN PDDDFRPALK DTKLLKTLAI GDTITSQELL AQAQSILNKN HPGYTIYERD SSIVTHDNDI FRTILPMDQE FTYHVKNREQ AYRINKKSGL NEEINNTDLI SEKYYVLKKG EKPYDPFDRS HLKLFTIKYV DVNTNELLKS EQLLTASERN LDFRDLYDPR DKAKLLYNNL DAFGIMDYTL TGKVEDNHDD TNRIITVYMG KRPEGENASY HLAYDKDRYT EEEREVYSYL RYTGTPIPDN PNDK |
| 产品编号 | 产品名称 | 包装 |
| LM7046-20μg | Recombinant SK | 20μg |
| LM7046-100μg | Recombinant SK | 100μg |
| LM7046-1mg | Recombinant SK | 1mg |
| - | 说明书 | 1份 |
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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文献和实验1. Inoculate the entire colony in 5 ml YPD culture O.N. or 12 hours 2. Dilute into YPA medium (1% yeast extract, 2% Bacto-peptone, 2%KOAC) to OD600~ 0.2, Vigorous shaking. 3. For ts mutants, ~15hours at 23C, others ~10 hours at 30C
适用标准:GB/T3536-91(克力夫兰开口杯法)GB/T267-88 产品说明: 本仪器采用当代先进技术,集机械、光学,电子及计算机技术于一体,结构紧凑。可自动完成石油产品开口闪点的测试,实验过程中的升温速率及扫描点火动作由微机控制自动进行,油气闪火时,检测系统自动捕捉并记录闪点温度。对闪点数据进行自动气压修正,并以规范的格式打印输出,还可根据需要重复打印数据。 本仪器可由计算机监控(无线/有线通讯方式,由用户选配)。 本仪器结构合理,性能稳定,操作简单,是理想的分析
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
-ligate ), the antibiotic resistance gene will allow bacteria carrying empty vector plasmids to survive the selection.Transformation The reaction used to create recombinant DNA molecules (ligation reaction, etc.) is used to transform bacteria
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