Alpha-N-acetylgalactosaminide
alpha-2, 6-sialyltransferase 2 (ST6GALNAC2) ELISA Kit, Human- $975
- KAMIYA BIOMEDICAL COMPANY
- 详见说明书
- KT-62178
- 美国加利福尼
- 2025年07月14日
14 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 样本：
血清、血浆、尿液、组织、上清液
- 库存：
60
- 标记物：
详见官方网站
- 适应物种：
Human
- 应用：
ELISA
- 检测方法：
酶联免疫方法
- 检测范围：
详见说明书
- 供应商：
钰博生物
- 规格：
96 tests
Alpha-N-acetylgalactosaminide alpha-2, 6-sialyltransferase 2 (ST6GALNAC2) ELISA Kit, Human
| For quantitative detection in human samples. | (no photo available) | 96 tests | $975 | KT-62178 |
COMPONENTS
| Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 |
| Calibrator | 2 |
| Calibrator Diluent | 1 × 20 mL |
| Detection Reagent A | 1 × 120 uL |
| Detection Reagent B | 1 × 120 uL |
| Assay Diluent A | 1 × 12 mL |
| Assay Diluent B | 1 × 12 mL |
| TMB Substrate | 1 × 9 mL |
| Stop Solution | 1 × 6 mL |
| Wash Buffer (30X concentrate) | 1 × 20 mL |
| Plate sealer for 96 wells | 4 |
- Microplate reader with 450 ± 10 nm filter.
- Precision single or multi-channel pipettes and disposable tips.
- Eppendorf Tubes for diluting samples.a
- De-ionized or distilled water.
- Absorbent paper for blotting the microtiter plate.
- Container for Wash Solution. STORAGE All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon being received while the others should be at 4°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable for 1 month, provided it is stored as prescribed above.
Plasma
Collect plasma using 3.8% sodium citrate (sodium citrate:blood = 1:9) as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g at 4°C within 30 minutes of collection. Remove plasma and assay immediately or store
samples in aliquot at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles. Note:
- Samples to be used within 5 days may be stored at 4°C, otherwise samples must be stored at -20°C (≤1 month) or -80°C (≤2 months) to avoid loss of bioactivity and contamination.
- When performing the assay slowly bring samples to room temperature. 3. Sample hemolysis will influence the result, so hemolytic specimen can not be detected.
Bring all kit components and samples to room temperature (18-25°C) before use.
Detection Reagent A and B
Briefly spin or centrifuge the stock Detection Reagent A and Detection Reagent B before use. Dilute to the working concentration with Assay Diluent A or B, respectively (1:100).
Wash Solution
Dilute 20 mL of Wash Solution concentrate (30X) with 580 mL of de-ionized or distilled water to prepare 600 mL of Wash Solution (1X).
ASSAY PROCEDURE SUMMARY
- Prepare all reagents, samples and calibrators;
- Add 100 µL calibrator or sample to each well. Incubate 2 hours at 37°C;
- Add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
- Aspirate and wash 3 times;
- Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
- Aspirate and wash 5 times;
- Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37°C;
- Add 50 µL Stop Solution. Read at 450 nm immediately.
1. The final experimental results will be closely related to operation skills of the end users and theexperimental environments. Please make sure that sufficient samples are available.
2. Kits from different batches may be a little different in detection range, sensitivity and color developingtime. Please perform the experiment exactly according to the instruction attached in kit whileelectronic ones from our website (www.k-assay.com) is only for information.
3. Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied bymanufacturer.
4. Protect all reagents from strong light during storage and incubation. All the bottle caps of reagentsshould be covered tightly to prevent the evaporation and contamination of microorganism.
5. There may be some foggy substance in the wells when the plate is opened at the first time. It will nothave any effect on the final assay results. Do not remove microtiter plate from the storage bag untilneeded.
6. Wrong operations during the reagents preparation and loading, as well as incorrect parameter settingfor the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10 nmor less and an optical density range of 0-3 O.D. or greater at 450 ± 10 nm wavelength is acceptablefor use in absorbance measurement. Please read the instruction carefully and adjust the instrumentprior to the experiment.
7. Even the same operator might get different results in two separate experiments. In order to get betterreproducible results, the operation of every step in the assay should be controlled. Furthermore, apreliminary experiment before assay for each batch is recommended.
8. Each kit has been strictly passed Q.C. test. However, results from end users might be inconsistentwith our in-house data due to some unexpected transportation conditions or different lab equipments.
Intra-assay variance among kits from different batches might arise from above factors, too.
9. Kits from different manufacturers for the same item might produce different results, since we haven’tcompared our products with other manufacturers.
10. The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, andclothing protection when using this material.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验相关实验
一篇文献中的ELISA方法如下：Control peptides (14–17 amino acid sequence near the N-terminal or C-terminal of the studied protein) and the reactive rabbit anti-defensin antibodies (affinity pure) were obtained from Alpha Diagnostics .CoStar flat-bottom high
CANDOR BIOSCIENCE: 关于ELISA板稳定性的技术探讨与比较
部分。它们的错误折叠，如天然结构的丢失，是任何分析的一个重大问题，因为它会引起抗体结合部位、抗体的抗原结合区或抗原的表位的改变，这两者都是待测物结合所必需的。因此，捕获分子无法结合待测物，甚至可能由于暴露天然未暴露的氨基酸序列和结构而发生非特异性结合，导致诊断试验显示错误的结果。由于表面效应引起的空间结构和构象的改变，抗体在包被过程中会发生错误折叠。在ELISA微孔板中，只有3-10%的包被抗体具有良好的定向性和与待测物结合的功能活性。抗原包被也有类似的情况。在大多数情况下，很少量的活性成分就足以完成一次检测
结构不同，这决定了它们的抗原性也不同。IgG 和IgM 的重链分别称为γ(gamma )链和μ(mu )链。重链和轻链的 N 端的氨基酸排列顺序因各种抗体而异，称为可变区，分别用VH 和VL 表示。两者构成抗体的抗原结合部位，只与相应的抗原决定簇匹配，发生特异性结合，是抗体专一性结合抗原的结构基础。 IgG 可被木瓜蛋白酶分解为三个区段，其中两个相同的区段称抗原 结合片段(Fab )。每个Fab 都保存结合抗原的能力，但只有一个抗原结合位点，是单价的，与抗原结合后不出
技术资料暂无技术资料 索取技术资料
Alpha-N-acetylgalactosaminide alpha-2, 6-sialyltransferase 2 (ST6GALNAC2) ELISA Kit, Human
$975
