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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 英文名：
Annexin-V-FLUOS Staining Kit
- 供应商：
上海善然生物科技有限公司
- 保存条件：
低温
General description下载特定产品批次和规格的CofA、SDS和IFU。 In the early stages of apoptosis, changes occur at the cell surface. One of these plasma membrane alterations is the translocation of phosphatidylserine (PS) from the inner side of the plasma membrane to the outer layer, exposing PS at the surface of the cell. Macrophages specifically recognize PS exposed on the cell surface of lymphocytes during the development of apoptosis. The recognition and phagocytosis of apoptotic cells and bodies protects organisms from exposure to cellular compounds that lead to inflammation, which usually accompanies necrosis. Annexin V is a 35.8kDa, Ca2+ -dependent phospholipid-binding protein with a high affinity for PS, making labeled annexin an excellent detection agent.[1][2]
Detection: Annexin-V-FLUOS can be directly detected in FACS analysis and immunochemistry without a secondary detection system.
Sample material: Cell lines and freshly isolated cells.
Kit with FLUOS-conjugated annexin-V and propidium iodide for the detection and quantification of apoptosis and differentiation from necrosis at the single-cell level.Other Notes仅用于生命科学研究。不可用于诊断。包装1 kit containing 3 componentsPreparation NoteWorking solution: Predilute 20 μl Annexin-V-FLUOS labeling reagent in 1 ml Incubation buffer and add 20 μl Propidium iodide solution.
Note: 1 ml is enough for 10 samples.
Storage conditions (working solution): Diluted staining solution should be always prepared freshly.SpecificityAnnexin-V-FLUOS binds in a Ca2+-dependent manner to negatively charged phospholipid surfaces, and shows high specificity for phosphatidylserine. Therefore, it stains apoptotic and necrotic cells. Propidium iodide stains only the DNA of leaky necrotic cells and allows for a distinction between apoptotic and necrotic cells.ApplicationLabeled annexin-V, with its high affinity for phosphatidylserine (PS), is a sensitive probe for PS exposed on the outer layer of apoptotic cells. Since necrotic cells also expose PS as a result of lost membrane integrity, propidium iodide is utilized as a DNA stain to distinguish necrotic cells from annexin-V-labeled cell clusters. Other secondary labeling is possible, such as membrane-surface staining with phycoerythrin- or TRITC-labeled monoclonal antibodies for further cellular characterization.[3][4][5
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文献和实验磷脂酰丝氨酸（Phosphatidylserine, PS）正常位于细胞膜的内侧，但在细胞凋亡的早期，PS可从细胞膜的内侧翻转到细胞膜的表面，暴露在细胞外环境中（图3）。Annexin-V是一种分子量为35~36KD的Ca2+依赖性磷脂结合蛋白，能与PS高亲和力特异性结合。将Annexin-V进行荧光素（FITC、PE）或biotin标记，以标记了的Annexin-V作为荧光探针，利用流式细胞仪或荧光显微镜可检测细胞凋亡的发生。碘化丙啶（propidine iodide, PI）是一种核酸
Annexin V是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸PS有高度亲和力，可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合，将Annexin V标记上荧光染料利用流式细胞仪或荧光显微镜可检测细胞凋亡。 由于凋亡晚期或坏死细胞膜完整性丧失，细胞核染色液可进入细胞内染色细胞核，搭配Annexin V使用可将处于不同凋亡时期的细胞区分开。 A．悬浮细胞： 按照实验方案进行凋亡诱导，300 g离心5 min，弃上清，收集细胞，用PBS洗涤细胞一次，轻轻重悬浮细胞并计数。 取1~5 ×
磷脂酰丝氨酸（Phosphatidylserine, PS）正常位于细胞膜的内侧，但在细胞凋亡的早期，PS可从细胞膜的内侧翻转到细胞膜的表面，暴露在细胞外环境中。Annexin-V是一种分子量为35~36KD的Ca2+依赖性磷脂结合蛋白，能与PS高亲和力特异性结合。将Annexin-V进行荧光素（FITC、PE）或biotin标记，以标记了的Annexin-V作为荧光探针，利用流式细胞仪或荧光显微镜可检测细胞凋亡的发生。 碘化丙啶(propidine iodide
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