- ¥1152
- 联迈生物
- LM6002-10μg
- 中国/上海
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Recombinant Murine G-CSF
- 保质期：
一年
- 供应商：
上海联迈生物工程有限公司
- 保存条件：
-20℃或更低温度保存
- 规格：
10μg
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| LM6002-10μg | Recombinant Murine G-CSF | 10μg | 1152.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Mouse | 12985 | LM09920 | E. coli | 178aa | 18.9kDa | － |
| About this Antibody | |
| Name | Recombinant Murine G-CSF (Recombinant Murine Granulocyte Colony Stimulating Factor; rMuG-CSF)；Recombinant Mouse G-CSF ; 重组小鼠粒细胞集落刺激因子 |
| Synonyms | CSF3; C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3OS; filgrastim; Filgrastim; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin; Pluripoietin |
| Purity | >98% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05ng/ml, corresponding to a specific activity of >2.0×107IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in 10mM Sodium Citrate, pH4.0, 150mM NaCl, 0.01% Tween-20. |
| Endotoxin | Less than 0.1EU/μg of rMuG-CSF as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Cytokine |
| Background | Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is mainly produced by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Besides, many other cell types can secreted this protein after LPS, IL-1 or TNF-α activation, which are fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF is cytokine that acts in hematopoiesis by controlling the production, differentiation and function of 2 related white cell populations of the blood, the granulocytes and the monocytesmacrophages. In addition, it may function in some adhesion or recognition events at the cell surface. The murine G-CSF cDNA encodes a 208 amino acid (a.a.) residue precursor protein containing a 30a.a. residue signal peptide that is proteolytically cleaved to generate the 178a.a. residue mature protein. Murine G-CSF is 73% identical at the amino acid level to human G-CSF and the two proteins show species cross-reactivity. |
| Amino Acid Sequence | VPLVTVSALP PSLPLPRSFL LKSLEQVRKI QASGSVLLEQ LCATYKLCHP EELVLLGHSL GIPKASLSGC SSQALQQTQC LSQLHSGLCL YQGLLQALSG ISPALAPTLD LLQLDVANFA TTIWQQMENL GVAPTVQPTQ SAMPAFTSAF QRRAGGVLAI SYLQGFLETA RLALHHLA |
| 产品编号 | 产品名称 | 包装 |
| LM6002-2μg | Recombinant Murine G-CSF | 2μg |
| LM6002-10μg | Recombinant Murine G-CSF | 10μg |
| LM6002-100μg | Recombinant Murine G-CSF | 100μg |
| LM6002-1mg | Recombinant Murine G-CSF | 1mg |
| - | 说明书 | 1份 |
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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