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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Recombinant Streptavidin
- 保质期:
一年
- 供应商:
上海联迈生物工程有限公司
- 保存条件:
-20℃或更低温度保存
- 规格:
1g
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| LM5084-1g | Recombinant Streptavidin | 1g | 12897.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| - | - | - | E. coli | - | - | - |
| About this Protein | |
| Name | Recombinant Streptavidin (Recombinant Streptavidin; rStreptavidin);重组链霉亲和素 |
| Synonyms | N/A |
| Purity | >98% by SDS-PAGE and HPLC analyses |
| Biological Activity | >17U/mg (one unit binds 1μg D-biotin at pH8.9) |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized in 10mM potassium phosphate buffer pH6.5. |
| Endotoxin | Less than 0.1EU/μg of rStreptavidin as determined by LAL method. |
| Reconstitution | Dissolve with double distilled water. |
| Category | Others |
| Background | Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1×10-15M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin. |
| Amino Acid Sequence | MAEAGITGTW YNQLGSTFIV TAGADGALTG TYESAVGNAE SRYVLTGRYD SAPATDGSGT ALGWTVAWKN NYRNAHSATT WSGQYVGGAE ARINTQWLLT SGTTEANAWK STLVGHDTFT KVKPSAAS |
| 产品编号 | 产品名称 | 包装 |
| LM5084-1mg | Recombinant Streptavidin | 1mg |
| LM5084-5mg | Recombinant Streptavidin | 5mg |
| LM5084-1g | Recombinant Streptavidin | 1g |
| - | 说明书 | 1份 |
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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文献和实验The overexpression of toxic recombinant proteins is often problematic, leading to either low production levels or inclusion bodies. Streptavidin is no exception and thus the highest production level reported to date for streptavidin is 70 mg
Colloidal Gold/Streptavidin Methods
. This nonspecific binding has been attributed both to its high isoelectric point (PI = 10) and to the fact that the protein is glycosylated (3 , 4 ). Because of the problem of nonspecific binding of avidin, streptavidin has largely replaced avidin
Smart Polymer-Streptavidin Conjugates
The conjugation of stimuli-responsive, or “smart,” polymers to streptavidin is described. The polymer is synthesized with a thiol-reactive end-group, which is used to end-graft the polymer to cysteine or lysine side-chains
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