- ¥4098
- 密理博
- IPVH00010
- 美国
- 2026年04月29日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保修期：
至少1年
- 现货状态：
长期现货
- 库存：
50
- 供应商：
北京孚博
- 规格：
26.5 cm x 3.75 m/卷
适用：Western blotting、吸附分析、氨基酸分析、N 端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析
替代原产品 INCP 000 10（蛋白质应用）
产品链接：https://www.merckmillipore.com/CN/zh/product/Immobilon-P-PVDF-Membrane,MM_NF-IPVH00010
产品详情：
| 背景信息 | Overview - Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications. Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times. Immobilon-P Transfer Membrane: - 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa - Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity Immobilon-E Membrane: - the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step. - 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa Blotting Sandwiches: - When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device. Blotting Filter Paper: - Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes. Immobilon-PSQ Membrane: - 0.2 µm pore size and a large internal structure - Higher protein adsorption and sequencing yields than other membranes - Recommended for blotting proteins less than 20 kDa - Prevents blow-through of low molecular weight proteins Immobilon-FL Membrane: - The first transfer membrane optimized for fluorescence applications - Extremely low background improves sensitivity of all fluorescence detection protocols - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths - Ideal for multiplexing and chemifluorescence applications Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications. Features & Benefits: - Won’t crack, curl or fracture when cut - Low background - Superior staining capabilities - Can be reprobed multiple times Applications: - Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing; - Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths |
| 产品信息 | |
|---|---|
| 检测方法 | 呈色 放射 |
| 相容的染色剂 |
|
| 过滤器代码 | IPVH |
| 过滤器颜色 | 白色 |
| 应用 | |
|---|---|
| 应用 | Western blotting、吸附分析、氨基酸分析、N 端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析 |
| 主要应用 |
|
| 生物信息 | |
|---|---|
| 主结合机制 | 静电，疏水 |
| BSA吸收性 | 215 µg/cm² |
| 山羊IgG吸收性 | 294 µg/cm² |
| 胰岛素吸收性 | 160 µg/cm² |
| 可湿润性 | 疏水 |
| 理化信息 | |
|---|---|
| 孔径 | 0.45 µm |
| 尺寸 | |
|---|---|
| 过滤器表面 | 平纹 |
| 过滤器尺寸 | 26.5 cm x 3.75 m |
| 材料信息 | |
|---|---|
| 化学 |
|
| 包装信息 | |
|---|---|
| 数量 | 1 |
了解更多产品请留言，或 直接与孚博生物销售人员联系
联系人： 邢先生 电话：13260236521 | 固话：010-56146401 | QQ：20493615 | Email：info@f-biology.com
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文献和实验Western Blotting 膜法学堂：选择正确的转印膜，优化
五万二千篇国际期刊的文献使用了Immobilon-P。对于一般的化学发光的免疫检测，使用Immobilon-P 型号就可以了。 但有些特殊情况需要选择特别的转印膜。转印膜的显微结构其实是疏松多孔的材质，膜的孔径大小和膜的厚度等参数会影响不同分子量蛋白质的结合量和截留率。Immobilon-PSQ 型号的PVDF膜由于具有更均一的孔径以及更厚的横截面，因此其内表面积是其它大部分膜的3倍，再加上Immobilon-PSQ 型号的膜孔径是更小的0.2 μm，所以Immobilon
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