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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 英文名：
KnockOut™ Serum Replacement - Multi-Species
- 供应商：
上海善然生物科技有限公司
- 保存条件：
低温
描述Gibco KnockOut Serum Replacement – Multi-Species (KnockOut SR – Multi-Species) is a more defined, FBS-free medium supplement that supports the growth of pluripotent stem cells (PSCs) cultured on fibroblast feeder cells. Designed to replace fetal bovine serum (FBS) in existing protocols, this Research Use Only product uses the same KnockOut Serum Replacement formulation that has been trusted by many for almost 20 years. For pre-clinical or clinical applications, please see Cat. No. 10828028.
With KnockOut Serum Replacement – Multi-Species, you can:
• Culture mouse, human, and other mammalian feeder-dependent PSCs with confidence
• Rely on the improved performance of a qualified, FBS-free formulation
• Enjoy the peace of mind of having a more stable price and supply compared to FBS
Culture mammalian PSCs with confidence
The KnockOut SR – Multi-Species formulation has been cited in more than 2000 publications. It has been shown to support feeder-dependent culture of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) from mouse, human, and other mammalian species. For mouse and human PSCs, it has also been shown to support reprogramming, embryoid body (EB) formation, in vitro differentiation, cryopreservation, ESC derivation, and many other applications.
Rely on improved performance
KnockOut SR – Multi-Species has a more defined formulation compared to FBS, which contains many undefined and variable components. Unlike most FBS products, each lot is tested for its ability to support PSCs. Because of this, culturing mouse and human PSCs with KnockOut SR – Multi-Species leads to less differentiation and more compact colony morphology compared to FBS.
Enjoy the peace of mind of having stable price and supply
KnockOut SR – Multi-Species is not affected by the limited supply and fluctuating prices often seen with FBS. This makes it easier to plan your budget and buy what you need, especially since it is more affordable than most FBS products.
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For Research Use Only. Not for use in diagnostic procedures.
Figures
Undifferentiated embryonic stem cell growth PSC marker expression in KSR – Multi-Species vs FBS PSC morphology in KSR – Multi-Species vs FBS Comparison of serum and the KSR formulation (used in KSR – Multi-Species) Human PSC growth in KSR – Multi-Species vs FBS
规格
Classification:Serum-FreeProduct Line:KnockOut Product Size:500 mLGreen Features:Sustainable packagingShipping Condition:Dry Ice
内容及储存
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文献和实验Gene Knockout In Murine Embryonic Stem (ES) Cells
When everything is set up then proceed to trypsinise ES cells as follows. Aspirate off the media, wash twice with MTPBS, add 0.5 ml trypsin versene with 1% chicken serum. Place at 37°C for no more than 3 minutes. At this point one can see that the cells have lifted
Adult cattle serum as supplemental nutrient in lymphocyte cultures for chromosomes studies
serum has been found to give desired mitotic division of lymphocytes of several animal species for chromosome evaluation. Adult cattle serum as supplemental nutrient in lymphocyte cultures for chromosomes studies B. R. Yadav
all different kinds of proteins to allow for high-density coating. However, this characteristic in turn causes the problem that many assay components, like antibodies from the serum sample, can also nonspecifically bind to this surface, leading to faulty
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