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- 2026年07月10日
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5 μmstainless steel
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文献和实验Determination of Molecular Size by Size‐Exclusion Chromatography (Gel Filtration)
Mobile phase for conventional SEC (see recipe ) Gel filtration support (Table 5.5.2 ) with appropriate fractionation range (see Strategic Planning
HPLC‐SEC Characterization of Membrane Protein‐Detergent Complexes
Figure 29.5.5 Optimization of HPLC‐SEC: impact of column pre‐equilibration with a buffer containing high detergent concentration. A Sepax SRT‐500 column, 7.8 × 300 mm, was used for fractionation. Dashed line
guideline is presented, based on the measured sedimentation coefficient and a calculated maximum S , to estimate if a protein is globular or elongated. It is recalled that a gel filtration column fractionates proteins on the basis of their Stokes radius
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