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文献和实验Determination of Molecular Size by Size‐Exclusion Chromatography (Gel Filtration)
Mobile phase for conventional SEC (see recipe ) Gel filtration support (Table 5.5.2 ) with appropriate fractionation range (see Strategic Planning
of the unknown protein SMC protein from B. subtilis was determined by gel filtration. Fig. 3 Determination of R s of BsSMC by gel filtration. The column was calibrated by running standard proteins BSA, catalase, and thyroglobulin
. Combine all Wnt-containing fractionsand pass through a 0.2-μm syringe filter. Fig. 2.3. Gel filtration of Wnt3A and b-catenin assay. The elution peak positions ofmolecular weight standards on the Superdex 200 column are indicated at the top. Wnt
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