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文献和实验are then purified and spotted onto poly-L-lysine coated microscope slides. In the second operation, BASIC PROTOCOL 2, RNA EXTRACTION AND LABELING, RNA is extracted from the cell samples to be examined, purified, and used as the substrate for reverse transcription
核蛋白基因组指纹技术Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells
medium (see below). The same procedure is used for yeast (Strahl-Bohlsinger et al. 1997). Adherent mammalian cells are also cross-linked by adding a concentrated stock of the cross-linking buffer to the medium. After cross-linking and quenching
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
structure. Growing Cells for Immunofluorescence We plate cells on glass coverslips (12mm circles or similar ). We pretreat coverslips typically with poly-L-lysine (PLL) if cells are loosely adherent to glass. Polyornithine is better for some neurons
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