Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.
Product Name:β-tubulin Mouse Monoclonal Antibody-HRP Conjugated
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:20,000-640,000 IHC: 1:1,000-2,000
Optimal dilutions should be determined by the end user. Avoid using SODIUM AZIDE.
Specificity: Theβ-tubulin antibody can detects endogenousβ-tubulin protein.
Alternative Names:Beta 4 tubulin, TBB5, TUBB2, TUBB2A, tubulin beta 2A, beta tubulin, tubulin beta chain
Molecular Weight:55KD
Form: Liquid
Reactivity:H, R, M, Mk, Dg, C, Hm, Rb, Sh, Insect, Yeast
Western blot analysis of Hela, with β-tubulin-HRP Conjugated BE3312 diluted at 1:10,000, 20,000, 40,000, 80,000, 160,000, 320,000.