- ¥1480
- EASYBIO
- BE3312-100
- 2026年01月06日
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- 克隆性：
多克隆
- 规格：
100ul/支
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.
Product Name:β-tubulin Mouse Monoclonal Antibody-HRP Conjugated
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:20,000-640,000 IHC: 1:1,000-2,000
Optimal dilutions should be determined by the end user. Avoid using SODIUM AZIDE.
Specificity: Theβ-tubulin antibody can detects endogenousβ-tubulin protein.
Alternative Names:Beta 4 tubulin, TBB5, TUBB2, TUBB2A, tubulin beta 2A, beta tubulin, tubulin beta chain
Molecular Weight:55KD
Form: Liquid
Reactivity:H, R, M, Mk, Dg, C, Hm, Rb, Sh, Insect, Yeast
Western blot analysis of Hela, with β-tubulin-HRP Conjugated BE3312 diluted at 1:10,000, 20,000, 40,000, 80,000, 160,000, 320,000.
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文献和实验Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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