- ¥1480
- EASYBIO
- BE7005-100
- 2026年01月05日
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V5-HRP Mouse Monoclonal Antibody
V5 tag Mouse mAb is the excellent antibody in the research. V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein. V5 Tag Antibody can recognize C-terminal, internal, and N-terminal V5-tagged proteins.
Product Name: V5-tag mouse monoclonal antibody-HRP conjugated
Clone Number: 4C9
Isotype: IgG1
Immunogen: A synthetic peptide GKPIPNPLLGLDST coupled to KLH.
Tested applications and dilutions:WB 1:2,000-5,000
Produce anti- V5 antibody affinity column purify V5 recombinant proteins
Form: Liquid
Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: V5 antibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Western blot analysis of V5 recombinant protein with V5 Mouse mAb(Q31) diluted at
1:5,000
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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
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