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- 详细信息
- 文献和实验
- 技术资料
- 库存:
631
- 英文名:
Recombinant Mouse Neutrophil gelatinase-associated lipocalin
- 保质期:
6个月
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
收到货后请置于-20℃
特别声明:本产品及我公司所售其他产品均为科研类试剂产品,严禁用于药物、医疗及其他非科研用途。
北京百奥莱博供应的JN1471型重组小鼠Lipocalin-2(NGAL)(C端含His标签)大量库存促销用于生化科研试验,本产品质量好,且极具价格优势,深为用户称道,了解更多重组小鼠Lipocalin-2(NGAL)(C端含His标签)等重组蛋白产品请联*(代"系")我司咨询订购。
名称:JN1471型重组小鼠Lipocalin-2(NGAL)(C端含His标签)大量库存促销
品牌:百奥莱博
英文名:Recombinant Mouse Neutrophil gelatinase-associated lipocalin
编号:JN1471
规格:10μg|50μg|500μg|1mg
产地:国产|进口
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Gln21-Asn200在C端含有His标签。
Lipocalin-2/NGAL 质量控制:>95%(还原性SDS-PAGE)
Lipocalin-2/NGAL 制剂:液体
Lipocalin-2/NGAL 保存:收到货后请置于-20℃,可保存6个月,避免反复冻融。
关于Lipocalin-2/NGAL :
Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), is a secretory protein of the lipocalin superfamily. Lipocalin-2 contains a signal peptide that enables it to be secreted and form complexes with matrix metalloproteinase-9 (MMP-9) through disulfide bonds. Similar to other lipocalin family members, Lipocalin-2 is involved in diverse cellular processes, including the transport of small hydrophobic molecules, protection of MMP-9 from proteolytic degradation, and cell signaling. Furthermore, Lipocalin-2 can tightly bind to bacterial siderophore through a cell surface receptor, possibly serving as a potent bacteriostatic agent by sequestering iron, regulating innate immunity and protecting kidney epithelial cells from ischemia–reperfusion injury. This protein is mainly expressed in neutrophils and in lower levels in the kidney, prostate, and epithelia of the respiratory and alimentary tracts. Recent evidence also suggests its role as a biomarker for renal injury and inflammation.
欲了解更多JN1471型重组小鼠Lipocalin-2(NGAL)(C端含His标签)大量库存促销的品牌、产地、价格及说明书等产品信息,请致电北京百奥莱博科技有限公司,我们将竭诚为您服务,另外,以下产品正在火爆促销:
·重组人IL-2Rα(C端带Fc标签)
编号:JN0413
英文名称:Recombinant Human Interleukin-2 Receptor subunit alpha
规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物表达系统制备,目的基因编码的Glu22-Cys213在N端含有Fc标签。
IL-2RA/CD25质量控制:>95%(还原性SDS-PAGE)。
IL-2RA/CD25制剂:冻干品
IL-2RA/CD25保存:
冻干蛋白可在-20℃长期保存,室温也可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天。
复溶后建议分装冻存在-20℃,可保存3个月,应避免反复冻融。
IL-2RA/CD25复溶:
1、试剂开盖前请先离心。
2、复溶浓度应大于100 μg/ml.
3、冻干蛋白溶于ddH2O中
4、复溶后根据使用量分装保存,避免反复冻融。
关于IL-2RA/CD25:
Interleukin-2 receptor subunit alpha (IL2RA) is a single-pass type I membrane protein, contains 2 Sushi (CCP/SCR) domains. The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis.
JN1471型重组小鼠Lipocalin-2(NGAL)(C端含His标签)大量库存促销关键词:重组小鼠NGAL,JN1471,重组小鼠Lipocalin-2,重组小鼠Lipocalin-2蛋白,C端含His标签
·重组小鼠IL-1 RII
编号:JN1571
英文名称:Recombinant Mouse Interleukin-1 Receptor Type 2
规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Phe14-Glu355在C端带有Fc标签。
IL-1 RII质量控制:>95%(还原性SDS-PAGE)
IL-1 RII制剂:冻干品
IL-1 RII保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
IL-1 RII复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100 μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于IL-1 RII:
Mouse Interleukin 1 receptor, type II (IL1R2) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL-1R2 structurally consisting of a ligand binding portion comprised of three Ig-like domains, a single transmembrane region, and a short cytoplasmic domain. It is expressed in a variety of cell types including B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Mouse IL1RII shares 59% amino acid sequence homology with human IL1 RII in their extracellular domains. The pleiotropic cytokine IL1 is produced to regulate development and maintenance of the inflammatory responses, and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling, whereas the IL-1R2 has no signaling property and acts as a decoy for IL-1.
JN1471型重组小鼠Lipocalin-2(NGAL)(C端含His标签)大量库存促销关键词:重组小鼠NGAL,JN1471,重组小鼠Lipocalin-2,重组小鼠Lipocalin-2蛋白,C端含His标签
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北京百莱博科技有限公司专业生产重组蛋白产品,欢迎来电咨询选购JN1471型重组小鼠Lipocalin-2(NGAL)(C端含His标签)大量库存促销。
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文献和实验。当某一个标签的使用,一是能构成表位利于纯化和检测;二是构成独特的结构特征(结合配体)利于纯化。组氨酸残基侧链与固态的镍有强烈的吸引力,可用于固定化金属螯合层析(IMAC),对重组蛋白进行分离纯化。使用His-tag有下面优点:1.标签的分子量小,只有~0.84KD,而GST和蛋白A分别为~26KD和~30KD,一般不影响目标蛋白的功能;2.His标签融合蛋白可以在非离子型表面活性剂存在的条件下或变性条件下纯化,前者在纯化疏水性强的蛋白得到应用,后者在纯化包涵体蛋白时特别有用,用高浓度的变性剂溶解
激活结构域(transcriptional activation domain, AD)。这两个结构域即使分开时仍各具功能,互不影响。但一个完整的某个特定基因的转录激活因子必须同时含有这两个结构域,否则无法完成激活功能。只有将这两部分通过适当的途径在空间上接近才能恢复其激活转录的能力。不同来源的BD与AD结合后则特异地激活BD结合基因的表达。基于这一特性,Fields等设计了一个检测蛋白质与蛋白质相互作用的系统。选择的转录激活因子是酵母细胞中的GAL4蛋白。分离GAL4蛋白N端的1-147个氨基酸(BD)和C端的768-881个氨
一个或一个以上的选择性标记基因(如抗生素抗性基因)和一个人工合成的含有多个限制性内切酶识别位点的多克隆位点序列,并去掉了大部分非必需序列,使分子量尽可能减少,以便于基因工程操作。大多质粒载体带有一些多用途的辅助序列,这些用途包括通过组织化学方法肉眼鉴定重组克隆、产生用于序列测定的单链DNA、体外转录外源DNA序列、鉴定片段的插入方向、外源基因的大量表达等。一个理想的克隆载体大致应有下列一些特性:(1)分子量小、多拷贝、松驰控制型;(2)具有多种常用的限制性内切酶的单切点;(3)能插入较大的外源
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