[ PROPERTIES ]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific Affinity Chromatography.
Traits: Liquid
Concentration: 200μg/mL
UOM: 100μg
Applications: WB; IHC; ICC; IP
[ APPLICATIONS ]
Western blotting: 0.5-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.
[ FORMULATION ]
Form & Buffer: Supplied as solution form in 0.01M PBS, pH7.4, containing
0.05% Proclin-300, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant ADAM33 disposed in loading
buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per we ll wh en used in enhan ced ch emil umes cen t (E C L) .
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol,
0.02% BPB, 50mM DTT and 0.02% NaN3.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 4oC for frequent use.
Aliquot and store at -20oC for 12 months