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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
biorbyt
- 检测范围:
6.25-400 ng/mL
- 检测方法:
Sandwich
- 应用:
ELISA
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
2.45 ng/mL
- 规格:
10 x 96 T
产品描述:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inter Alpha-Globulin Inhibitor H3(ITIH3).
应用说明:standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITIH3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITIH3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITIH3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ITIH3 in the samples is then determined by comparing the OD of the samples to the standard curve
检测时间:3.5h
Note:For research use only.
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., Ectopic expression of OsLFL1 in rice represses Ehd1 by binding on its promoter. Biochem Biophys Res Commun, 2007. 360(1): p. 251-6. [14] Omata, K., et al., Identification and characterization of the human inhibitor of caspase-activated DNase gene promoter
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TSA Treatment of Mammalian Cells
Introduction Prolonged treatment of proliferating mammalian cells with low doses of a histone deacetylase inhibitor, trichostatin A, specifically affects pericentric heterochromatin. Relocation of these regions towards the nuclear
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