HIV-1 Protease

HIV-1 Protease

CATALOGUE NUMBER

HIV-001

INTRODUCTION

HIV-1 protease is very significant in the life cycle of the HIV virus. It is expressed in the infected cells as a part of Gag-Pol polyprotein from which it is auto-catalytycaly released after formation of an immature viral particle. The enzyme subsequently cleaves the other parts of viral polyproteins resulting in the maturation of the virus. In HIV-infected patients the enzyme is subjected to intensive mutagenesis and mutants resistant to applied medicines are produced as a result of the selection pressure.

DESCRIPTION

HIV-1 protease is an active homodimer having a molecular mass of 21.6kDa (each monomer of 99 amino acids is 10.8kDa).

SOURCE

Escherichia Coli.

PHYSICAL APPEARANCE

Sterile filtered colorless clear solution.

FORMULATION

The HIV-1 Protease filtered (0.4µm) solution is formulated in 20mM Tris, 20mM MES, 200mM NaCl, 1mM EDTA, 10% (v/v) glycerol and 0.05% 2-mercaptoethanol, pH 6.5.

STABILITY

Store at 4°C if entire vial will be used within 2-4 weeks. 
Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles. 

PURITY

Greater than 95.0% as determined by SDS-PAGE.

KINETIC PARAMETERS

Km=15.1µM, Kcat = 30s-1, Kcat/Km= 1981 mM-1s-1 with peptide substrate KARVF (NO2)VRKA (F(NO2) ... p-nitrophenylalanine).

AMINO ACID SEQUENCE

PQITLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMNLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF.

USAGE

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.