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- 克隆性:
多克隆
- 抗体英文名:
human CD56-PE
- 规格:
25T
中文名称PE标记小鼠抗人CD56单克隆抗体
别 名CD56 antigen; Cell Adhesion Molecule Neural 1; MSK39; N CAM 120; N CAM 140; N CAM 180; NCAM 1; NCAM 120; NCAM 140; NCAM1; Neural cell adhesion molecule 1 120 kDa isoform; Neural cell adhesion molecule 1 140 kDa isoform; Neural cell adhesion molecule 1; NCAM1_HUMAN. PE anti-human CD56;
规格25T 100T
研究领域细胞生物 神经生物学 干细胞 细胞类型标志物
抗体来源Mouse
克隆类型Monoclonal
交叉反应 Human,
产品应用Flow-Cyt=20ul/Test (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量92kDa
细胞定位细胞膜 分泌型蛋白
性 状Liquid
免 疫 原:
亚 型IgG
纯化方法affinity purified by Protein G
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]
Function:
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Subcellular Location:
Isoform 1, 2,: Cell membrane; Single-pass type I membrane protein. Isoform 3, 4: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 5, 6: Secreted.
Similarity:
Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
SWISS:
P13591
Gene ID:
4684
Database links:
Entrez Gene: 4684 Human
Omim: 116930 Human
SwissProt: P13591 Human
Unigene: 503878 Human
Unigene: 711235 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PE标记小鼠抗人CD56单克隆抗体
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文献和实验步:选择抗体 在弹出的界面中列出了所有已选抗原可选的荧光素种类,并且在每一个通道内仅可选择一个抗原。 点击其中一种抗原与荧光素搭配,即可查看可供选择的所有货号信息,这里以抗CD56抗体偶联PE为例,在跳出的窗口中可以对比可选货号的信息,这里建议选择克隆号以REA开头的REAfinityTM重组抗体,比传统的单克隆抗体有更高的特异性和可重复性,点击select即可将选择保存至配色方案中。 依次在每个通道中选择需要的抗体即可完成配色。再次点击右上方的Continue,即可得到一份可以下载保存
于细胞阳选,也能用于细胞阴选中。 为了评估 Strep-Tactin®TACS 琼脂糖柱细胞阴选的效率,实验从外周血单个核细胞(PBMC)样品中富集了 CD3- CD56+自然杀伤细胞(NK)。材料为Strep-Tactin®TACS 琼脂糖柱以及生物素化的抗体,仅 10 分钟,便完成了细胞阴选(图 1)。 流程如下: 阴选流程:生物素化抗体与 PBMC 孵育标记非目的细胞,加入至亲和层析柱后,即可收集目的细胞 实验方案 CD3- CD56+ NK 的细胞阴选 · 将 1 x
恶性肿瘤病人外周血免疫指标的流式细胞仪检测及免疫治疗前后免疫功能变化的研究
,其中年龄和性别构成比与病人组差异无显著性(P>0.05)。 1.2 材料与仪器 小鼠抗人单克隆抗体CD4/CD8/CD3、CD19、CD20、CD3/CD16+56、CD3/HLA-DR、CD3/CD25及同型对照、红细胞裂解液(Optilyse C)均为法国Immunotech公司出品。流式细胞仪(Epics XL·MCL)是美国BECKMAN COULTER公司生产。 1.3 研究方法 使用直接免疫荧光标记全血溶血法,流式细胞仪测定肿瘤病人外周血淋巴细胞表面抗原表达。
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