C-藻蓝蛋白(C-PC)在许多蓝细菌中作为主要的藻胆蛋白发生,在一些红藻中作为次生的藻胆蛋白发生。该颜料在615和620nm之间具有单个可见吸收最大值,在~650nm处具有最大荧光发射。其分子量在70,000至110,000道尔顿之间。颜料由两个亚基α和β组成,它们以相同的数量出现,但构成分子的α和β对的确切数目可能因物种而异。α和β亚基都仅含有PCB发色团。 除了直接吸收光之外,这种强烈的蓝色颜料通过荧光能量转移接受来自藻红蛋白的量子,其中存在PE的生物体。C-PC的红色荧光转移到别藻蓝蛋白。AAT Bioquest研发的RPE、APC试剂及试剂盒被国内外科研人员灵活运用,达到不俗的科研成果。
Upgrade: ReadiUse™ CL-APC [Cross linked-Allophycocyanin] *Ammonium Sulfate-Free*
See also: PE and APC
Molecular weight 105000
Extinction coefficient (cm -1 M -1) 700000
Excitation (nm) 651
Emission (nm) 660
Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. Allophycocyanin is the least stable among the major phycobiliproteins, susceptible to dissociation at low concentrations including concentrations at which some assays are performed. CL-APC is chemically cross-linked between ? and β subunits, and is much more stable than APC. The crosslinked allophycocyanin has improved stability in aqueous solution.
Calculators
Common stock solution preparation
Table 1. Volume of appropriate solvent needed to reconstitute specific mass of CL-APC [Cross linked-AlloPhycocyanin] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg
0.5 mg
1 mg
5 mg
10 mg
1 mM
952.381 nL
4.762 µL
9.524 µL
47.619 µL
95.238 µL
5 mM
190.476 nL
952.381 nL
1.905 µL
9.524 µL
19.048 µL
10 mM
95.238 nL
476.19 nL
952.381 nL
4.762 µL
9.524 µL
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.