A2M Human

A2M Human

CATALOGUE NUMBER

PRO-551

SYNONYMS

Alpha-2-macroglobulin, Alpha-2-M, A2M, CPAMD5, FWP007, S863-7, alpha 2M, DKFZp779B086.

INTRODUCTION

Alpha-2 Macroglobulin is a serine proteases inhibitor, which inhibits coagulation by inactivating thrombin and Kallikrein, it inhibits fibrinolysis by inactivating plasmin and involved in transport. Alpha-2-Macroglobulin is a large plasma protein, which is produced by the liver, it’s composed of 4 identical subunits bound together by -S-S- bonds. A2M is able to inactivate many kinds of proteinases (including serine-, cysteine-, aspartic- and metalloproteinases). A2M has a 35 amino acid "bait" region in its structure. Proteinases bind and cleave the “bait” region become bound to A2M. Macrophage receptors recognize the proteinase-A2M complex and clear it from the system. A2M binds to and removes MMP-2 and MMP-9 (active forms of the gelatinase) from the circulation using scavenger receptors on the phagocytes. The levels of Alpha-2-macroglobulin are increased in nephrotic syndrome which is a condition where the kidneys start to leak out some of the smaller blood proteins. Due to its large size, A2-macroglobulin is retained in the bloodstream. Increased production of all proteins causes A2-macroglobulin concentration to increase. Chronic renal failure might lead to amyloid by alpha-2-macroglobulin. A2M is raised in cirrhosis, pregnancy and diabetes.

DESCRIPTION

Human Alpha-2 Macroglobulin is a tetrameric glycoprotein, produced in Human plasma and having a molecular mass of 725 kDa.

SOURCE

Human Plasma.

PHYSICAL APPEARANCE

Sterile Filtered White lyophilized (freeze-dried) powder.

FORMULATION

Lyophilized from 0.02M TRIS, 0.15M NaCl and 0.1M Sucrose, pH 8.0.

SOLUBILITY

It is recommended to reconstitute the lyophilized A2M in sterile 18MΩ-cm H₂O.

STABILITY

Human Alpha-2 Macroglobulin although stable at room temperature for 3 weeks, should be stored between 2-8°C. Do not freeze!

HUMAN VIRUS TEST

Serum from each donor has been tested and found negative for antibody to HIV-1, HIV-2, HCV, HBSAG.

PURITY

Greater than 96.0%.

USAGE

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.