CD45 leukocyte common antigen (LCA) belongs to the family of at least four isoforms of membrane glycoproteins (220, 205, 190, 180kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portion of these molecules has protein phosphatase activity and is involved in regulation of transmembrane signals.
CLONE
hCD45.
IMMUNOGEN
Purified human T-Cells.
IG SUBCLASS
Mouse IgG2a.
PURIFICATION METHOD
Protein-A.
FORMULATION
1mg/ml in PBS (after reconstitution).
STORAGE PROCEDURES
Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.
SOLUBILITY
Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
APPLICATIONS
Staining antibody. For staining, use 10µl/1,000,000 cells.
AVAILABLE CONJUGATES
This antibody is also available unconjugated and Biotin conjugated. For staining with biotin or FITC-conjugated antibody use 5-10µl/106 cells.
USAGE
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.