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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 英文名:
Mouse Glucose Colorimetric Detection Kit
- 保质期:
十二个月
- 供应商:
信裕生物
- 保存条件:
-20°C
- 规格:
96 tests
Mouse Glucose Colorimetric Detection Kit
Catalog No.: IRAAKT2557
$350
Category: Assay and ELISA Kits
Product Description
Mouse Glucose Colorimetric Detection Kit
The Glucose Colorimetric Detection Kit is designed to quantitatively measure glucose in a variety of samples. Please read the complete kit insert before performing this assay. A -D-glucose standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with the colorimetric substrate and horseradish peroxidase and the reaction initiated by addition of glucose oxidase. The reaction is incubated at room temperature for 30 minutes. The glucose oxidase reacts with glucose to produce hydrogen peroxide which, in the presence of HRP, reacts with the colorimetric substrate to convert the colorless substrate into a pink-colored product. The pink product is read at 560 nm. Increasing
levels of glucose cause a linear increase in color.
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intensity was measured at 22 min. The glucose oxidase assay shows a linear response over the tested range (Figure 3). This same method could be used in a study of enzymatic response to various conditions or in a detection test for glucose itself.
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