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- 2026年02月02日
- WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- 详见说明书
- 详见说明书
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Ras样蛋白A抗体
- 抗体英文名：
Anti-RALA
- 靶点：
详见说明书
- 浓度：
1mg/1ml
- 应用范围：
WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- 宿主：
详见说明书
- 供应商：
上海一研
- 库存：
59
- 级别：
详见说明书
- 目录编号：
详见说明书
- 抗原来源：
Rabbit
- 保质期：
详见说明书
- 适应物种：
详见说明书
- 标记物：
详见说明书
- 克隆性：
多克隆
- 保存条件：
Store at -20 °C
- 形态：
详见说明书
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human RalA
- 规格：
0.2ml/200μg
公司抗体应用于WB、IHC、IF、ELISA等实验中，Ras样蛋白A抗体品牌品质保证，提供完善的售前、售中、售后服务！欢迎来电咨询或联系在线客服！我们将竭诚为您服务！
英文名称 Anti-RALA
中文名称 Ras样蛋白A抗体品牌
别 名 Ral a; Ral A protein; RAL; RALA; RALA_HUMAN; RAS like protein A; Ras related protein RalA; Ras-related protein Ral-A.
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit
产品类型 一抗
研究领域 肿瘤 细胞生物 免疫学 信号转导 G蛋白偶联受体
蛋白分子量 predicted molecular weight: 24kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human RalA
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Multifuntional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells.
Function : Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells.
Subunit : Interacts with RALBP1 via its effector domain. Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have overlapping binding sites and compete for RALA binding. Interacts with Clostridium exoenzyme C3. Interacts with RALGPS1. Interacts with LPAR1 and LPAR2. Interacts with ADRBK1 in response to LPAR1 activation. RALA and ADRBK1 mutually inhibit each other's binding to LPAR1.
Subcellular Location : Cell surface. Cell membrane; Lipid-anchor; Cytoplasmic side. Cleavage furrow. Midbody.
Post-translational modifications : Prenylation is essential for membrane localization. The geranylgeranylated form and the farnesylated mutant does not undergo alternative prenylation in response to geranylgeranyltransferase I inhibitors (GGTIs) and farnesyltransferase I inhibitors (FTIs).
Similarity : Belongs to the small GTPase superfamily. Ras family.
Database links : UniProtKB/Swiss-Prot:
Ras样蛋白A抗体品牌具有高度的特异性，免疫组织化学正是利用了这一原理。先将组织或细胞中的某种化学物质提取出来，以此作为抗原或半抗原，通过免疫动物后获得特异性的抗体，再以此抗体去探测组织或细胞中的同类的抗原物质。由于抗原与抗体的复合物是无色的，因此还必须借助于组织化学的方法将抗原抗体结合的部位显示出来，以其达到对组织或细胞中的未知抗原进行定性，定位或定量的研究。
Ras样蛋白A抗体品牌包括以下几个步骤：
1、制备抗原。
2、选择实验动物。
3、动物免疫。
4、试取血进行测试，看看是否成功免疫。
5、如果成功免疫，杀死实验动物，采集全部血清。
6、纯化出抗体。
7、鉴定抗体。包括纯度以及特异性。
Ras样蛋白A抗体品牌的功能
抗体的主要功能是与抗原（包括外来的和自身的）相结合，从而有效地清除侵入机体内的微生物、寄生虫等异物，抗体（antibody）是一种应答抗原产生的、可与抗原特异性结合的蛋白质。每种抗体与特定的抗原决定基结合。这种结合可以使抗原失活，也可能无效但有时也会对机体造成病理性损害，如抗核抗体、抗双链DNA抗体、抗甲状腺球蛋白抗体等一些自身抗体的产生，对人体可造成危害。
Ras样蛋白A抗体品牌的优点：
①耐热、耐酸、耐碱、抗有机溶剂以及金属离子，稳定性好，室温下可以长期保存；
②制备简单，操作方便，可以进行批量；
③不必免疫动物，且可获得免疫动物所不能得到的“抗体”；
④可以反复使用；
⑤价格低廉。因此，设计、合成既具有类似生物抗体的高亲合性和高专一性，又具有耐热、耐酸、耐碱且又可以长期稳定的人工抗体，在部分领域替代生物抗体以进行仿生分子识别，或者完成一些生物抗体所不能完成的工作，具有重要的科学意义，在化学、生命科学和环境科学等方面具有广阔的应用前景
公司专业供应各种进口、国产一抗及二抗，品牌有R&D、Santa Cruz、Bipec、Millipore等国际知名品牌，品种多达7000多种，由于产品的数量众多，产品的宿主、抗原来源及应用范围(WB、IH(F/P)、FC、ELISA、IP、IF等)有所不同。
GI4含亮氨酸神经胶质瘤失活蛋白4抗体规格:0.1ml
SESN2/Hi95缺氧诱导基因95抗体规格:0.2ml
BTNL2嗜脂蛋白样2抗体规格:0.2ml
Lingo-1Nogo受体反应蛋白抗体规格:0.1ml
KAT13A/SRC1类固醇受体激活蛋白1抗体规格:0.2ml
NDUFA1NADH氧化还原酶辅酶1抗体规格:0.2ml
Phospho-NPM(Thr95)磷酸化核仁磷酸蛋白抗体规格:0.1ml
GLUR4谷氨酸受体4抗体规格:0.1ml
phospho-Rb/p105-Rb(Ser780)磷酸化成视网膜细胞瘤抑蛋白抗体规格:0.1ml
SDHD线粒体琥珀酸脱酶D抗体规格:0.2ml
Sca1/Ly6A/ET淋巴细胞激活蛋白抗体规格:0.2ml
Smad7/Smad6Smad7抗体规格:0.1mlQ-K-R-P-S-Q-R-S-K-Y-L Gln-Lys-Arg-Pro-Ser-Gln-Arg-Ser-Lys-Tyr-Leu
Selectin Cys-Gln-Lys-Leu-Asp-Lys-Ser-Phe-Ser-Met-Ile-Lys
Substance P (6-11)//Hexa-Substance P Gln-Phe-Phe-Gly-Leu-Met-NH2
Tyrosinase (192-200) (human, mouse) Ser-Glu-Ile-Trp-Arg-Asp-Ile-Asp-Phe
Xenopsin-Related Peptide 1 (XP-1) His-Pro-Lys-Arg-Pro-Trp-Ile-Leu
Ras样蛋白A抗体品牌β- MSH, porcine Asp-Glu-Gly-Pro-Tyr-Lys-Met-Glu-His-Phe-Arg-Trp-Gly-Ser-Pro-Pro-Lys-Asp
β-Endorphin (27-31) (human) Tyr-Lys-Lys-Gly-Glu
[Cys2, Tyr3, Orn5, Pen7-amide]-Somatostatin 14 (7-14) D-Phe-Cys-Tyr-D-Trp-Orn-Thr-Pen-Thr-NH2
[Des-Pro2] Bradykinin Arg-Pro-Gly-Phe-Ser-Pro-Phe-Arg
[Gln22] -β- Amyloid (1-40) Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Gln-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val
[Nle11] Substance P Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Nle-NH2
[Trp11] Neurotensin (8-13) Arg-Arg-Pro-Trp-Ile-Leu
[Val4]-Angiotensin III Arg-Val-Tyr-Val-His-Pro-Phe
Ac-Endothelin-1 (16-21), human Ac-His-Leu-Asp-Ile-Ile-Trp
Adipokinetic Hormone, G (AKH-G) Pyr-Val-Asn-Phe-Ser-Thr-Gly-Trp-NH2
英文名称 Anti-RALA
中文名称 Ras样蛋白A抗体品牌
别 名 Ral a; Ral A protein; RAL; RALA; RALA_HUMAN; RAS like protein A; Ras related protein RalA; Ras-related protein Ral-A.
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit
产品类型 一抗
研究领域 肿瘤 细胞生物 免疫学 信号转导 G蛋白偶联受体
蛋白分子量 predicted molecular weight: 24kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human RalA
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Multifuntional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells.
Function : Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells.
Subunit : Interacts with RALBP1 via its effector domain. Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have overlapping binding sites and compete for RALA binding. Interacts with Clostridium exoenzyme C3. Interacts with RALGPS1. Interacts with LPAR1 and LPAR2. Interacts with ADRBK1 in response to LPAR1 activation. RALA and ADRBK1 mutually inhibit each other's binding to LPAR1.
Subcellular Location : Cell surface. Cell membrane; Lipid-anchor; Cytoplasmic side. Cleavage furrow. Midbody.
Post-translational modifications : Prenylation is essential for membrane localization. The geranylgeranylated form and the farnesylated mutant does not undergo alternative prenylation in response to geranylgeranyltransferase I inhibitors (GGTIs) and farnesyltransferase I inhibitors (FTIs).
Similarity : Belongs to the small GTPase superfamily. Ras family.
Database links : UniProtKB/Swiss-Prot:
Ras样蛋白A抗体品牌具有高度的特异性，免疫组织化学正是利用了这一原理。先将组织或细胞中的某种化学物质提取出来，以此作为抗原或半抗原，通过免疫动物后获得特异性的抗体，再以此抗体去探测组织或细胞中的同类的抗原物质。由于抗原与抗体的复合物是无色的，因此还必须借助于组织化学的方法将抗原抗体结合的部位显示出来，以其达到对组织或细胞中的未知抗原进行定性，定位或定量的研究。
Ras样蛋白A抗体品牌包括以下几个步骤：
1、制备抗原。
2、选择实验动物。
3、动物免疫。
4、试取血进行测试，看看是否成功免疫。
5、如果成功免疫，杀死实验动物，采集全部血清。
6、纯化出抗体。
7、鉴定抗体。包括纯度以及特异性。
Ras样蛋白A抗体品牌的功能
抗体的主要功能是与抗原（包括外来的和自身的）相结合，从而有效地清除侵入机体内的微生物、寄生虫等异物，抗体（antibody）是一种应答抗原产生的、可与抗原特异性结合的蛋白质。每种抗体与特定的抗原决定基结合。这种结合可以使抗原失活，也可能无效但有时也会对机体造成病理性损害，如抗核抗体、抗双链DNA抗体、抗甲状腺球蛋白抗体等一些自身抗体的产生，对人体可造成危害。
Ras样蛋白A抗体品牌的优点：
①耐热、耐酸、耐碱、抗有机溶剂以及金属离子，稳定性好，室温下可以长期保存；
②制备简单，操作方便，可以进行批量；
③不必免疫动物，且可获得免疫动物所不能得到的“抗体”；
④可以反复使用；
⑤价格低廉。因此，设计、合成既具有类似生物抗体的高亲合性和高专一性，又具有耐热、耐酸、耐碱且又可以长期稳定的人工抗体，在部分领域替代生物抗体以进行仿生分子识别，或者完成一些生物抗体所不能完成的工作，具有重要的科学意义，在化学、生命科学和环境科学等方面具有广阔的应用前景
公司专业供应各种进口、国产一抗及二抗，品牌有R&D、Santa Cruz、Bipec、Millipore等国际知名品牌，品种多达7000多种，由于产品的数量众多，产品的宿主、抗原来源及应用范围(WB、IH(F/P)、FC、ELISA、IP、IF等)有所不同。
GI4含亮氨酸神经胶质瘤失活蛋白4抗体规格:0.1ml
SESN2/Hi95缺氧诱导基因95抗体规格:0.2ml
BTNL2嗜脂蛋白样2抗体规格:0.2ml
Lingo-1Nogo受体反应蛋白抗体规格:0.1ml
KAT13A/SRC1类固醇受体激活蛋白1抗体规格:0.2ml
NDUFA1NADH氧化还原酶辅酶1抗体规格:0.2ml
Phospho-NPM(Thr95)磷酸化核仁磷酸蛋白抗体规格:0.1ml
GLUR4谷氨酸受体4抗体规格:0.1ml
phospho-Rb/p105-Rb(Ser780)磷酸化成视网膜细胞瘤抑蛋白抗体规格:0.1ml
SDHD线粒体琥珀酸脱酶D抗体规格:0.2ml
Sca1/Ly6A/ET淋巴细胞激活蛋白抗体规格:0.2ml
Smad7/Smad6Smad7抗体规格:0.1mlQ-K-R-P-S-Q-R-S-K-Y-L Gln-Lys-Arg-Pro-Ser-Gln-Arg-Ser-Lys-Tyr-Leu
Selectin Cys-Gln-Lys-Leu-Asp-Lys-Ser-Phe-Ser-Met-Ile-Lys
Substance P (6-11)//Hexa-Substance P Gln-Phe-Phe-Gly-Leu-Met-NH2
Tyrosinase (192-200) (human, mouse) Ser-Glu-Ile-Trp-Arg-Asp-Ile-Asp-Phe
Xenopsin-Related Peptide 1 (XP-1) His-Pro-Lys-Arg-Pro-Trp-Ile-Leu
Ras样蛋白A抗体品牌β- MSH, porcine Asp-Glu-Gly-Pro-Tyr-Lys-Met-Glu-His-Phe-Arg-Trp-Gly-Ser-Pro-Pro-Lys-Asp
β-Endorphin (27-31) (human) Tyr-Lys-Lys-Gly-Glu
[Cys2, Tyr3, Orn5, Pen7-amide]-Somatostatin 14 (7-14) D-Phe-Cys-Tyr-D-Trp-Orn-Thr-Pen-Thr-NH2
[Des-Pro2] Bradykinin Arg-Pro-Gly-Phe-Ser-Pro-Phe-Arg
[Gln22] -β- Amyloid (1-40) Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Gln-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val
[Nle11] Substance P Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Nle-NH2
[Trp11] Neurotensin (8-13) Arg-Arg-Pro-Trp-Ile-Leu
[Val4]-Angiotensin III Arg-Val-Tyr-Val-His-Pro-Phe
Ac-Endothelin-1 (16-21), human Ac-His-Leu-Asp-Ile-Ile-Trp
Adipokinetic Hormone, G (AKH-G) Pyr-Val-Asn-Phe-Ser-Thr-Gly-Trp-NH2
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文献和实验相关实验
／GDP交换因子(guanine exchange factor，GEF)，RalGDS激活RalA／B相关小GTP酶。RalBP，是一个GTP酶激活蛋白，它能抑制Cdc42和RacGTP酶，然后通过Rac／Cdc42调控激动蛋白细胞骨架的重组及转录因子NF―xB的活化，从而促进抗凋亡蛋白的产生来抑制细胞凋亡。 Ras信号转导通路 注：IKK：IκB激酶；ELK―l：ETS样蛋白―1；ERK：细胞外信号调节激酶；MEK．．MAPK／ERK激酶； caspase：半胱氨酸天冬
。但做反相HPLC检测，纯度只有85%左右。由于两种检测方法的原理不同，样品中可能含有一些小分子的杂质，电泳检测不到，而反相HPLC可以检出。还有一个可能，就是酶切毕竟是个蛋白酶的水解过程，特异性再好也有错切的时候。也许有少量的蛋白A被多降解了几个氨基酸。另外就是目标蛋白质本身可能有高级结构方面的差异。回想起来，那可是一段沮丧的日子。***尚未成功，同志仍需努力。 （6）SP Sepharose FF柱纯化 pH5.8，20mM PB平衡SP柱，Q柱穿透样小心调节pH5.8后上样SP柱，目标
0.8um的膜或双层滤纸过滤。多数层析的要求都是过柱溶液需经过0.45um的膜过滤，但实际上对破菌后的上清液或包涵体的溶解上清样，0.45um膜太难过滤了，mission impossible，无法完成操作。所以在实际工作中，我们都是采用双层滤纸或0.8um的膜过滤。在此推荐我用的一套设备组合：millipore wp6122050型台式真空压力两用泵和Sartorius 16201不锈钢过滤器，非广告，好用哦。五、包涵体处理 前面说过对纯化来说，我不赞成包涵体的提法，但为了表述方便还是不妨借用一下
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