Intercellular Adhesion Molecule-1 Human Recombinant, SF9
保质期:
1年
供应商:
上海沪震实业有限公司
保存条件:
-20°C
规格:
2μg/10μg/1mg
ICAM1 Human, Sf9
CATALOGUE NUMBER
PRO-2200
SYNONYMS
Intercellular Adhesion Molecule 1, Major Group Rhinovirus Receptor, ICAM-1, Intercellular Adhesion Molecule 1 (CD54), Human Rhinovirus Receptor, Cell Surface Glycoprotein P3.58, Human Rhinovirus Receptor, CD54 Antigen, P3.58, CD54, BB2, Intercellular adhesion molecule 1.
INTRODUCTION
ICAM-1 also called CD54 is a single chain membrane glycoprotein expressed on the surface of a variety of non-haematopoietic and haematopoietic cell types and has roles in signal transduction, cell signaling and lymphocyte adhesion. ICAM1 binds to integrins such as CD11a / CD18, or CD11b / CD18. ICAM1 is also used by Rhinovirus as a receptor. ICAM-1 is an intercellular adhesion molecule constantly present in low concentrations in the membranes of leukocytes and endothelial cells. When stimulated by cytokine the concentrations significantly increase. ICAM-1 can be stimulated by interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFA) and is expressed by the vascular endothelium, macrophages and lymphocytes. ICAM-1 is a ligand for LFA-1 which is a receptor found on leukocytes. Upon activation, leukocytes bind to endothelial cells via ICAM-1/LFA-1 and then transmigrate into tissues. ICAM-1 is implicated in subarachnoid hemorrhage (SAH). Levels of ICAM-1 are shown to be notably elevated in patients with SAH. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory conditions.
DESCRIPTION
ICAM1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 692 amino acids (28-480a.a.) and having a molecular mass of 76.5kDa. (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).
ICAM1 is expressed with a 239 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
SOURCE
Sf9, Baculovirus cells.
PHYSICAL APPEARANCE
Sterile Filtered colorless solution.
FORMULATION
ICAM1 protein solution (0.25mg/ml) contains phosphate buffered saline (pH7.4) and 10% glycerol.
STABILITY
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.