相关应用: Real Time PCR-Based Gene Expression Profiling | miRNA 和非编码 RNA 谱分析 | 实时定量 PCR
保质期:
1年
供应商:
上海恒斐生物
保存条件:
-20
规格:
1 x 5 mL
TaqMan™ Universal Master Mix II, with UNG 货号4440038
Applied Biosystems™ 相关应用: Real Time PCR-Based Gene Expression Profiling | miRNA 和非编码 RNA 谱分析 | 实时定量 PCR
TaqMan 描述
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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.
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TaqMan® Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). You can use TaqMan® Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.
This TaqMan® Universal Master Mix II is supplied as a 2X concentrate and contains:
Note: This TaqMan® Universal Master Mix II contains Uracil-N glycoslyase (UNG). See a complete listing of all TaqMan® Universal Master Mix II products, with and without UNG here.
TaqMan® Universal Master Mix II has been optimized for use with primers and TaqMan® probes that have been designed according to Life Technologies' guidelines. The master mix can be used with custom TaqMan® assays available from our custom assay service, or with pre-optimized assays such as:
• TaqMan® Gene Expression Assays
• TaqMan® MicroRNA Assays
• TaqMan® Drug Metabolism Genotyping Assays
• TaqMan® SNP Genotyping Assays
For RNA quantitation experiments, the TaqMan® Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Life Technologies.
规格
Detection Method:
Primer-Probe Detection
For Use With (Equipment):
7300 System, 7500 System, 7900HT System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Reaction Speed:
Standard
PCR Method:
qPCR
Sample Type (General):
DNA
Passive Reference Dye:
ROX (Pre-mixed)
Polymerase:
AmpliTaq Gold® DNA Polymerase
Primer-Probe Compatibility:
TaqMan®
Product Line:
TaqMan™
Product Size:
1 x 5 mL
Purity or Quality Grade:
UP (Ultra Pure)
Sample Type (Specific):
DNA (Genomic), cDNA
Shipping Condition:
Wet Ice
内容及储存
Store at 4°C
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).