The quantikine Canine Troponin I (Tn-Ⅰ) immunoassay is a 4.5 hour solid phase elisa designed to measure Canine Troponin I (Tn-Ⅰ) levels in cell culture supernates, serum, and plasma. It contains e. Coli-expressed recombinant Canine Troponin I (Tn-Ⅰ) and antibodies raised against recombinant Canine Troponin I (Tn-Ⅰ). This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural Canine Troponin I (Tn-Ⅰ) showed dose response curves that were parallel to the standard curves obtained using the recombinant quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural Canine Troponin I (Tn-Ⅰ)