5-羟色胺受体2A抗体

英文名称5HT2A Receptor
中文名称5-羟色胺受体2A抗体
别    名5-hydroxytryptamine receptor 2A; 5-hydroxytryptamine receptor; Serotonin receptor 2A; 5-HT-2A; 5-HT-2; HTR2A; Htr2a; 5 HT 2; 5HTR2A; 5 HT 2A; 5 HT2 receptor; 5 HT2A; 5 hydroxytryptamine receptor 2A; 5-HT-2; 5-HT-2A; 5-hydroxytryptamine 2A receptor; 5-hydroxytryptamine receptor 2A; 5HT2A_HUMAN; HTR 2; HTR 2A; HTR2; HTR2A.
 
Specific References  (1)     |     bs-1056R has been referenced in 1 publications.[IF=2.59] Sun, Tao, et al. "Antihypertensive effect of formononetin through regulating the expressions of eNOS, 5-HT< sub> 2A/1B receptors and ??< sub> 1-adrenoceptors in spontaneously hypertensive rat arteries."European journal of pharmacology (2013)..  Rat.  PubMed:23123056
规格50ul  100ul  200ul
研究领域神经生物学  通道蛋白  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, 
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test IF=1:200-800 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量52kDa
细胞定位细胞膜 
性    状Lyophilized or Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human 5HT2A Receptor:65-160/471 <Cytoplasmic>
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
5HT2A receptor is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. 5HT2A receptor belongs to the G-protein coupled receptor 1 family and mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production. 5HT2A receptor is an integral membrane protein which localizes to the post-synaptic thickening of axo-dendritic synapses. 5HT2A receptor protein contains a PDZ domain-binding motif which is involved in the interaction with INADL, CASK, APBA1, DLG1 and DLG4.

Function:
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production.

Subunit:
Interacts with MPDZ and INADL. May interact with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2.

Subcellular Location:
Cell membrane; Multi-pass membrane protein. Note=Localizes to the postsynaptic thickening of axo-dendritic synapses.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P28223

Gene ID:
3356

Database links:
Entrez Gene: 3356 Human
Entrez Gene: 15558 Mouse
Entrez Gene: 29595 Rat
Omim: 182135 Human
SwissProt: P28223 Human
SwissProt: P35363 Mouse
SwissProt: P14842 Rat
Unigene: 654586 Human
Unigene: 72630 Human
Unigene: 214351 Mouse
Unigene: 10294 Rat
Unigene: 146756 Rat


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
 

产品图片

Sample:  U251(Human) Cell Lysate at 30 ug BV2(Mouse) Cell Lysate at 30 ug Primary: Anti-5HT2A Receptor (bs-1056R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (bs-1056R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (bs-1056R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (bs-1056R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat lung tissue;4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-5HT2A Receptor Polyclonal Antibody, Unconjugated(bs-1056R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (bs-1056R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G- cy3) for 90 minutes, and DAPI for nuclei staining. Blank control: U-2OS.  Primary Antibody (green line): Rabbit Anti-5HT2A Receptor antibody (bs-1056R)  Dilution: 3μg /10^6 cells;  Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 3μg /test.  Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.